Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-29847 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TRPC6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, A549 membrane fraction extract. Predicted reactivity: Mouse (96%), Rat (95%), Bovine (96%), Guinea pig (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.84 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TRPC6 using 30 µg of A549 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a TRPC6 polyclonal antibody (Product # PA5-29847) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of TRPC6 was performed by separating 30 µg of A549 whole cell and membrane extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a TRPC6 Polyclonal Antibody (Product # PA5-29847) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of TRPC6 was performed in paraffin-embedded mouse cerebellum tissue using Green: VGAT Polyclonal Antibody (Product # PA5-27569) at a dilution of 1:500. Red: NF-H, a neuron marker, stained by NF-H antibody diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
