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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- NB600-648-100ug - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal Ribonuclease A Antibody
- Antibody type
- Polyclonal
- Description
- Ion exchange chromatography. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Ribonuclease A [Bovine pancreas]. Cross-reactivity against Ribonuclease A from other tissues and species may occur but have not been specifically determined.
- Reactivity
- Bovine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Ribonuclease A Antibody [NB600-648] - Lane 1: Ribonuclease A (Bovine Pancreas). Load: 50 ng per lane. Primary antibody: Ribonuclease A (Bovine Pancreas) Antibody at 1:1,000 overnight at 4C. Secondary antibody: HRP rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 16.5 kDa, 16.5 kDa for Ribonuclease A.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Ribonuclease A Antibody [NB600-648] - Analysis using Biotin conjugate of NB600-648. Lane 1: Ribonuclease A (Bovine Pancreas). Load: 50 ng per lane. Primary antibody: Rabbit anti-Ribonuclease A Antibody Biotin Conjugated at 1:1,000 overnight at 4C. Secondary antibody: HRP streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 16.5 kDa, 16.5 kDa for Ribonuclease A. Other band(s): Possible dimerization.
