PA1-9069

antibody from Invitrogen Antibodies

Targeting: MMP7 MPSL1, PUMP-1

Antibody data

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Validation data

MMP7 protein structure - PA1-9069 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot detection of MMP7 in human breast lysate using Product # PA1-9069.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot detection of MMP7 in human breast lysate using Product # PA1-9069.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot detection of MMP7 in human breast lysate using Product # PA1-9069.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-MMP7 Polyclonal Antibody (Product # PA1-9069) and a 27 kDa band corresponding to MMP7 was observed. Whole cell extracts (30 µg lysate) of HT-29 (Lane 1), HT-29 treated with Protein Transport Inhibitor (PTI) cocktail (1X, 4 hours), SK-BR-3 (Lane 3) and SK-BR-3 treated with Protein Transport Inhibitor (PTI) cocktail (1X, 4 hours) (Lane 4) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.03 µg/mL) and detected by chemiluminescence with Rabbit anti-Goat IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27014,1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). Relative expression was observed between HT-29 and SK-BR-3, a cell line which is reported to express MMP7 at very low levels.