PAB23094

antibody from Abnova Corporation

Targeting: RNF7 CKBBP1, ROC2, SAG

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB23094

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB23094, RRID:AB_11121002

Product name

RNF7 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant RNF7.

Antigen sequence

SLKKWNAVAMWSWDVECDTCAICRVQVMDACLRCQ
AENKQEDCVVVWGECNHSFHNCCMSLWVKQNNRCP
LCQQDWVVQRIGK


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with RNF7 polyclonal antibody (Cat # PAB23094) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with RNF7 polyclonal antibody (Cat # PAB23094) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney with RNF7 polyclonal antibody (Cat # PAB23094) shows strong cytoplasmic positivity in cells in tubules at 1:20-1:50 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)