PA5-57906

antibody from Invitrogen Antibodies

Targeting: RNF7 CKBBP1, ROC2, SAG

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-57906

Provider

Invitrogen Antibodies

Product name

RNF7 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Immunogen sequence: SLKKWNAVAM WSWDVECDTC AICRVQVMDA CLRCQAENKQ EDCVVVWGEC NHSFHNCCMS LWVKQNNRCP LCQQDWVVQR IGK Highest antigen sequence identity to the following orthologs: Mouse - 100%, Rat - 100%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 μL

Concentration

0.05 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RNF7 in control (vector only transfected HEK293T lysate) and RNF7 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a RNF7 Polyclonal Antibody (Product # PA5-57906).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of RNF7 in human cell line A-431 shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a RNF7 Polyclonal Antibody (Product # PA5-57906).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorecent analysis of RNF7 in human cell line A-431 using RNF7 Polyclonal Antibody (Product # PA5-57906). Staining shows localization to nucleoplasm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of RNF7 in human kidney using RNF7 Polyclonal Antibody (Product # PA5-57906) shows moderate cytoplasmic positivity in cells in tubules.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of RNF7 in human liver using RNF7 Polyclonal Antibody (Product # PA5-57906) shows moderate cytoplasmic positivity in hepatocytes.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of RNF7 in human lymph node using RNF7 Polyclonal Antibody (Product # PA5-57906) shows very weak positivity in germinal center cells as expected.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of RNF7 in human pancreas using RNF7 Polyclonal Antibody (Product # PA5-57906) shows weak cytoplasmic positivity in exocrine glandular cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of RNF7 in human lymph node using RNF7 Polyclonal Antibody (Product # PA5-57906) shows very weak positivity in germinal center cells as expected.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of RNF7 in human pancreas using RNF7 Polyclonal Antibody (Product # PA5-57906) shows weak cytoplasmic positivity in exocrine glandular cells.