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- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Flow cytometry [1]
- Other assay [3]
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- Product number
- 701287 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MMP13 Recombinant Rabbit Monoclonal Antibody (3H13L17)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 3H13L17
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Xanthohumol Attenuated Inflammation and ECM Degradation by Mediating HO-1/C/EBPβ Pathway in Osteoarthritis Chondrocytes.
GYY4137 Regulates Extracellular Matrix Turnover in the Diabetic Kidney by Modulating Retinoid X Receptor Signaling.
Distinct biological events generated by ECM proteolysis by two homologous collagenases.
Zhang M, Zhang R, Zheng T, Chen Z, Ji G, Peng F, Wang W
Frontiers in pharmacology 2021;12:680585
Frontiers in pharmacology 2021;12:680585
GYY4137 Regulates Extracellular Matrix Turnover in the Diabetic Kidney by Modulating Retinoid X Receptor Signaling.
Juin SK, Pushpakumar S, Sen U
Biomolecules 2021 Oct 7;11(10)
Biomolecules 2021 Oct 7;11(10)
Distinct biological events generated by ECM proteolysis by two homologous collagenases.
Solomonov I, Zehorai E, Talmi-Frank D, Wolf SG, Shainskaya A, Zhuravlev A, Kartvelishvily E, Visse R, Levin Y, Kampf N, Jaitin DA, David E, Amit I, Nagase H, Sagi I
Proceedings of the National Academy of Sciences of the United States of America 2016 Sep 27;113(39):10884-9
Proceedings of the National Academy of Sciences of the United States of America 2016 Sep 27;113(39):10884-9
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MMP13 was performed by loading 20 µg of HeLa (lane1), HEK-293 (lane2) and Jurkat (lane3) cell lysates using Novex®NuPAGE®4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (Product # LC5800), and iBlot® Dry Blotting System (Product # IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5 % skim milk for 1 hour at room temperature. MMP13 was detected at ~54 kDa using MMP13 Recombinant Rabbit Monoclonal Antibody (Product # 701287) at 1 µg-3 µg/mL in 2.5 % skim milk at 4°C overnight on a rocking platform. Goat anti-Rabbit IgG-HRP Secondary Antibody (Product # G-21234) at 1:5000 dilution was used and chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MMP-13 in MDAMB-231 lysate (30 µg) using a MMP-13 recombinant rabbit monoclonal antibody (Product # 701287) at a dilution of 2 µg/mL. Samples were detected using chemiluminescence (ECL). Results show a band at ~54 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MMP-13 in whole cell extracts from MDAMB-231 cells using a MMP-13 recombinant rabbit monoclonal antibody (Product # 701287) at a dilution of 2 µg/mL. Samples were detected using chemiluminescence (ECL). Results show a band at ~54kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MMP-13 in HeLa cells using a MMP-13 recombinant rabbit monoclonal antibody (Product # 701287) followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (Image B) and actin stained with Alexa Fluor 594 phalloidin (red) (image C). Image D is a composite image showing nuclear localization of MMP-13.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of MMP13 was done on HeLa cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Tritonª X-100 for 20 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were labeled with ABfinityª MMP13 Recombinant Rabbit Monoclonal Antibody (701287, red histogram) or with rabbit isotype control (pink histogram) at 2 µg-4 µg/million cells in 2.5% BSA. After incubation at room temperature for 2-3 hours, the cells were labeled with Alexa Fluor¨ 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune¨ Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 1 The gross observation, histomorphological examination, and immunofluorescence assays in the knee articular cartilage. (A) The gross observation and histomorphological examination (HE staining) of articular cartilage. (B) The immunofluorescence study of MMP-13 in cartilage. (C) The summary data of fluorescence intensity of MMP-13 in situ . All experiments were performed in triplicate and data are presented as the mean +- standard deviation. * p < 0.05 and ** p < 0.01. NC, negative control; Model, the model group; Low, the group treated with XH (5.64 mg/kg); High, the group treated with XH (16.9 mg/kg).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 4 XH attenuated ECM degradation in IL-1beta-treated chondrocytes. (A) The immunofluorescence study of MMP-13 in IL-1beta-treated chondrocytes. (B) The summary data of fluorescence intensity of MMP-13. (C) The proteins expression of MMP-3, MMP-13, ADAMTS-4, ADAMTS-5, collagen-II, and aggrecan were detected by western blotting. (D-I) were the quantified values of tested proteins. All experiments were performed in triplicate and data are presented as the mean +- standard deviation. * p < 0.05 and ** p < 0.01. NC, negative control; Low, XH (5 muM); High, XH (20 muM).
