Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Immunohistochemistry [2]
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- Product number
- AF511 - Provider product page
- Provider
- R&D Systems
- Product name
- Human MMP-13 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human MMP-13 in Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) MMP-3 and rhMMP-8 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P45452
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references ErbB2-enhanced invasiveness of H-Ras MCF10A breast cells requires MMP-13 and uPA upregulation via p38 MAPK signaling.
Advanced glycation end products increases matrix metalloproteinase-1, -3, and -13, and TNF-alpha in human osteoarthritic chondrocytes.
Chondrocyte hypertrophy and apoptosis induced by GROalpha require three-dimensional interaction with the extracellular matrix and a co-receptor role of chondroitin sulfate and are associated with the mitochondrial splicing variant of cathepsin B.
Cyclooxygenase-2-derived E prostaglandins down-regulate matrix metalloproteinase-1 expression in fibroblast-like synoviocytes via inhibition of extracellular signal-regulated kinase activation.
Cyclooxygenase-2-derived E prostaglandins down-regulate matrix metalloproteinase-1 expression in fibroblast-like synoviocytes via inhibition of extracellular signal-regulated kinase activation.
Yong HY, Kim IY, Kim JS, Moon A
International journal of oncology 2010 Feb;36(2):501-7
International journal of oncology 2010 Feb;36(2):501-7
Advanced glycation end products increases matrix metalloproteinase-1, -3, and -13, and TNF-alpha in human osteoarthritic chondrocytes.
Nah SS, Choi IY, Yoo B, Kim YG, Moon HB, Lee CK
FEBS letters 2007 May 1;581(9):1928-32
FEBS letters 2007 May 1;581(9):1928-32
Chondrocyte hypertrophy and apoptosis induced by GROalpha require three-dimensional interaction with the extracellular matrix and a co-receptor role of chondroitin sulfate and are associated with the mitochondrial splicing variant of cathepsin B.
Olivotto E, Vitellozzi R, Fernandez P, Falcieri E, Battistelli M, Burattini S, Facchini A, Flamigni F, Santi S, Facchini A, Borzi' RM
Journal of cellular physiology 2007 Feb;210(2):417-27
Journal of cellular physiology 2007 Feb;210(2):417-27
Cyclooxygenase-2-derived E prostaglandins down-regulate matrix metalloproteinase-1 expression in fibroblast-like synoviocytes via inhibition of extracellular signal-regulated kinase activation.
Pillinger MH, Rosenthal PB, Tolani SN, Apsel B, Dinsell V, Greenberg J, Chan ES, Gomez PF, Abramson SB
Journal of immunology (Baltimore, Md. : 1950) 2003 Dec 1;171(11):6080-9
Journal of immunology (Baltimore, Md. : 1950) 2003 Dec 1;171(11):6080-9
Cyclooxygenase-2-derived E prostaglandins down-regulate matrix metalloproteinase-1 expression in fibroblast-like synoviocytes via inhibition of extracellular signal-regulated kinase activation.
Pillinger MH, Rosenthal PB, Tolani SN, Apsel B, Dinsell V, Greenberg J, Chan ES, Gomez PF, Abramson SB
Journal of immunology (Baltimore, Md. : 1950) 2003 Dec 1;171(11):6080-9
Journal of immunology (Baltimore, Md. : 1950) 2003 Dec 1;171(11):6080-9
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- MMP-13 in Human Breast. MMP-13 was detected in immersion fixed paraffin-embedded sections of human breast array using Goat Anti-Human MMP-13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF511) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- MMP-13 in Human Ovarian Cancer Tissue. MMP-13 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using 15 µg/mL Goat Anti-Human MMP-13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF511) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.