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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- TA319371 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal HIF3 alpha antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal HIF3 alpha antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- Hif3a
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 1.2 mg/mL
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Supportive validation
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- OriGene (provider)
- Main image
- Experimental details
- WB using Anti-Hif3A antibody shows detection of a band ~72 kDa corresponding to mouse Hif3A (arrowhead). Approximately 10 ug of a CoCl treated 3T3 cell lysate (lane 1) and control 3T3 cell lysate (lane 2) were separated by 4-20% SDS-PAGE and transferred onto nitrocellulose.? Treatment of exponentially growing 3T3 cells with 130 ?M CoCl for 6 h at 37¡ã C effectively mimics hypoxia. Primary antibody was diluted to 1:1,600.?IRDye?800 conjugated Gt-a-Rabbit IgG [H&L] MX was used at 1:10000.
- Validation comment
- WB
