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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00064344-B02P - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00064344-B02P, RRID:AB_1237838
- Product name
- HIF3A purified MaxPab mouse polyclonal antibody (B02P)
- Antibody type
- Polyclonal
- Description
- Mouse polyclonal antibody raised against a full-length human HIF3A protein.
- Antigen sequence
MALGLQRARSTTELRKEKSRDAARSRRSQETEVLY
QLAHTLPFARGVSAHLDKASIMRLTISYLRMHRLC
AAGEWNQVGAGGEPLDACYLKALEGFVMVLTAEGD
MAYLSENVSKHLGLSQLELIGHSIFDFIHPCDQEE
LQDALTPQQTLSRRKVEAPTERCFSLRMKSTLTSR
GRTLNLKAATWKVLNCSGHMRAYKPPAQTSPAGSP
DSEPPLQCLVLICEAIPHPGSLEPPLGRGAFLSRH
SLDMKFTYCDDRIAEVAGYSPDDLIGCSAYEYIHA
LDSDAVSKSIHTLLSKGQAVTGQYRFLARSGGYLW
TQTQATVVSGGRGPQSESIVCVHFLISRVEETGVV
LSLEQTEQHSRRPIQRGAPSQKDTPNPGDSLDTPG
PRILAFLHPPSLSEAALAADPRRFCSPDLRRLLGP
ILDGASVAATPSTPLATRHPQSPLSADLPDELPVG
TENVHRLFTSGKDTEAVETDLDIAQDADALDLEML
APYISMDDDFQLNASEQLPRAYHRPLGAVPRPRAR
SFHGLSPPALEPSLLPRWGSDPRLSCSSPSRGDPS
ASSPMAGARKRTLAQSSEDEDEGVELLGVRPPKRS
PSPEHENFLLFPLSLSFLLTGGPAPGSLQDPSTPL
LNLNEPLGLGPSLLSPYSDEDTTQPGGPFQPRAGS
AQAD- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of HIF3A expression in transfected 293T cell line (H00064344-T02) by HIF3A MaxPab polyclonal antibody.Lane1:HIF3A transfected lysate(73.59 KDa).Lane2:Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of purified MaxPab antibody to HIF3A on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
