600-401-A30

antibody from Rockland Immunochemicals, Inc.

Targeting: ATR FRP1, MEC1, SCKL, SCKL1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

600-401-A30

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#600-401-A30, RRID:AB_2289645

Product name

Anti-Serine/Threonine-Protein Kinase ATR (RABBIT) Antibody - 600-401-A30

Antibody type

Polyclonal

Vial size

100 µl

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western blot using Rockland's anti-ATR antibody shows detection of ATR in HeLa cell nuclear extract (lane 1). Lane 2 shows negligible staining after pre-incubation of antibody with the immunizing peptide (50 ?g peptide for 1 h at room temperature followed by centrifugation). A 4-8% gradient gel was used for separation. Goat serum was used at 5% for blocking. The arrowhead corresponds to 301 kDa ATR when compared to MW markers (Lane M). The primary antibody was used at a 1:1,400 dilution.

Validation comment

Western Blot