A02941-1

antibody from Boster Biological Technology

Targeting: CASP4 ICE(rel)II, ICH-2, TX

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

A02941-1

Provider

Boster Biological Technology

Product name

Anti-Caspase 4/CASP4 Antibody Picoband™

Antibody type

Polyclonal

Description

Polyclonal antibody for CASPASE 4/CASP4 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: ELISA. Reactive species: Human. CASPASE 4/CASP4 information: Subcellular Localization: Cytoplasm, cytosol; Tissue Specificity: Widely expressed, including in keratinocytes and colonic and small intestinal epithelial cells (at protein level). Not detected in brain.

Reactivity

Human

Host

Rabbit

Vial size

100μg/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

IHC analysis of Caspase 4 using anti-Caspase 4 antibody (A02941-1). Caspase 4 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Caspase 4 Antibody (A02941-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image