- PA5-29788 - Invitrogen Antibodies CASP4 antibody

No submitted references: Submit reference

No comments: Submit comment

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Caspase 4 was performed by loading 35 µg of THP-1 lysate in reducing sample buffer onto a 4-15% Precast Protein Gels. Proteins were transferred to PVDF and then blocked in blocking buffer (TBST+5% non-fat milk) for one hour at room temperature. Caspase 4 was detected at approximately 43 kDa using a caspase 4 polyclonal antibody (Product # PA5-29788) at a dilution of 1:1000 overnight at 4°C on a rocking platform, followed by a Rabbit IgG Horseradish Peroxidase-conjugated Antibody at a dilution of 1:1,000 for one hour. Chemiluminescent detection was performed using SuperSignal™ West Pico Chemiluminescent Substrate (Product # 34080).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of Caspase 4 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Caspase 4 Polyclonal Antibody (Product # PA5-29788) at a dilution of 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of Caspase 4 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Caspase 4 Polyclonal Antibody (Product # PA5-29788) at a dilution of 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using Caspase 4 Polyclonal Antibody (Product # PA5-29788). Sample (30 µg of whole cell lysate). Lane A: H1299. 10% SDS PAGE. Caspase 4 Polyclonal Antibody (Product # PA5-29788) diluted at 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-Caspase 4 Polyclonal Antibody (Product # PA5-29788) and a 45 kDa band corresponding to Caspase 4 was observed across all the cell lines tested. Whole cell extracts (30 µg lysate) of THP-1 (Lane 1) which is highly expressing as reported, Raw 264.7 (Lane 2), U-937 (Lane 3) which is highly expressing as reported, U-2 OS (Lane 4) which is medium expressing as reported, HeLa (Lane 5) which is Low expressing as reported and HEK-293 (Lane 6) which is low expressing as reported were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry (Paraffin) analysis of Caspase 4 was performed in paraffin-embedded human cervical carcinoma tissue using Caspase 4 Polyclonal Antibody (Product # PA5-29788) at a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Caspase 4 Polyclonal Antibody detects CASP4 protein at cytosol on SAS xenograft by immunohistochemical analysis. Sample: Paraffin-embedded SAS xenograft. Caspase 4 Polyclonal Antibody (Product # PA5-29788) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

PA5-29788