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- Product number
- NB120-10454 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB120-10454, RRID:AB_2259600
- Product name
- Rat Monoclonal Caspase-11 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse
- Host
- Rat
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references CircHIPK3 Promotes Pyroptosis in Acinar Cells Through Regulation of the miR-193a-5p/GSDMD Axis.
IRF8 Regulates Gram-Negative Bacteria-Mediated NLRP3 Inflammasome Activation and Cell Death.
Structural Mechanism for GSDMD Targeting by Autoprocessed Caspases in Pyroptosis.
IRF3 and IRF7 contribute to diesel exhaust particles-induced pulmonary inflammation by mediating mTORC1 activation and restraining autophagy in mice.
mtDNA Activates cGAS Signaling and Suppresses the YAP-Mediated Endothelial Cell Proliferation Program to Promote Inflammatory Injury.
Inflammasome Activation Induces Pyroptosis in the Retina Exposed to Ocular Hypertension Injury.
E3 ubiquitin ligase Nedd4 is a key negative regulator for non-canonical inflammasome activation.
Gasdermin D in peripheral myeloid cells drives neuroinflammation in experimental autoimmune encephalomyelitis.
IRF2 transcriptionally induces GSDMD expression for pyroptosis.
Entamoeba histolytica-induced IL-1β secretion is dependent on caspase-4 and gasdermin D.
Macrophage β2-Integrins Regulate IL-22 by ILC3s and Protect from Lethal Citrobacter rodentium-Induced Colitis.
Trimetazidine Attenuates Cardiac Dysfunction in Endotoxemia and Sepsis by Promoting Neutrophil Migration.
Guanylate binding proteins facilitate caspase-11-dependent pyroptosis in response to type 3 secretion system-negative Pseudomonas aeruginosa.
Copper Regulates the Canonical NLRP3 Inflammasome.
cGAS drives noncanonical-inflammasome activation in age-related macular degeneration.
Caspase-11-mediated endothelial pyroptosis underlies endotoxemia-induced lung injury.
Lipopolysaccharide-primed heterotolerant dendritic cells suppress experimental autoimmune uveoretinitis by multiple mechanisms.
Differential roles of caspase-1 and caspase-11 in infection and inflammation.
Differential Sensitivity of Target Genes to Translational Repression by miR-17~92.
Obesity-associated NLRC4 inflammasome activation drives breast cancer progression.
IRGB10 Liberates Bacterial Ligands for Sensing by the AIM2 and Caspase-11-NLRP3 Inflammasomes.
NLRP3 regulates a non-canonical platform for caspase-8 activation during epithelial cell apoptosis.
SESN2/sestrin2 suppresses sepsis by inducing mitophagy and inhibiting NLRP3 activation in macrophages.
Canonical Inflammasomes Drive IFN-γ to Prime Caspase-11 in Defense against a Cytosol-Invasive Bacterium.
Caspase-11 cleaves gasdermin D for non-canonical inflammasome signalling.
Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death.
The ketone metabolite β-hydroxybutyrate blocks NLRP3 inflammasome-mediated inflammatory disease.
Escherichia coli α-hemolysin counteracts the anti-virulence innate immune response triggered by the Rho GTPase activating toxin CNF1 during bacteremia.
Molecular mechanisms of cell death: central implication of ATP synthase in mitochondrial permeability transition.
Activation of the NLRP1b inflammasome independently of ASC-mediated caspase-1 autoproteolysis and speck formation.
Caspase-11 is expressed in the colonic mucosa and protects against dextran sodium sulfate-induced colitis.
Triggering receptor expressed on myeloid cells 1 (TREM-1)-mediated Bcl-2 induction prolongs macrophage survival.
FADD and caspase-8 mediate priming and activation of the canonical and noncanonical Nlrp3 inflammasomes.
Caspase-11 stimulates rapid flagellin-independent pyroptosis in response to Legionella pneumophila.
MicroRNA-17~92 plays a causative role in lymphomagenesis by coordinating multiple oncogenic pathways.
Microglial cathepsin B contributes to the initiation of peripheral inflammation-induced chronic pain.
An inactivating caspase 11 passenger mutation originating from the 129 murine strain in mice targeted for c-IAP1.
Non-canonical inflammasome activation targets caspase-11.
Distinct downstream pathways of caspase-11 in regulating apoptosis and cytokine maturation during septic shock response.
Dual role of caspase-11 in mediating activation of caspase-1 and caspase-3 under pathological conditions.
Dual role of caspase-11 in mediating activation of caspase-1 and caspase-3 under pathological conditions.
Wang J, Li X, Liu Y, Peng C, Zhu H, Tu G, Yu X, Li Z
Frontiers in medicine 2020;7:88
Frontiers in medicine 2020;7:88
IRF8 Regulates Gram-Negative Bacteria-Mediated NLRP3 Inflammasome Activation and Cell Death.
Karki R, Lee E, Sharma BR, Banoth B, Kanneganti TD
Journal of immunology (Baltimore, Md. : 1950) 2020 May 1;204(9):2514-2522
Journal of immunology (Baltimore, Md. : 1950) 2020 May 1;204(9):2514-2522
Structural Mechanism for GSDMD Targeting by Autoprocessed Caspases in Pyroptosis.
Wang K, Sun Q, Zhong X, Zeng M, Zeng H, Shi X, Li Z, Wang Y, Zhao Q, Shao F, Ding J
Cell 2020 Mar 5;180(5):941-955.e20
Cell 2020 Mar 5;180(5):941-955.e20
IRF3 and IRF7 contribute to diesel exhaust particles-induced pulmonary inflammation by mediating mTORC1 activation and restraining autophagy in mice.
Wang C, Li Y, Wu X, Tian H, Jiang S, Xu T, Liu Z, Sun JL, Qi X
European journal of immunology 2020 Mar 5;
European journal of immunology 2020 Mar 5;
mtDNA Activates cGAS Signaling and Suppresses the YAP-Mediated Endothelial Cell Proliferation Program to Promote Inflammatory Injury.
Huang LS, Hong Z, Wu W, Xiong S, Zhong M, Gao X, Rehman J, Malik AB
Immunity 2020 Mar 17;52(3):475-486.e5
Immunity 2020 Mar 17;52(3):475-486.e5
Inflammasome Activation Induces Pyroptosis in the Retina Exposed to Ocular Hypertension Injury.
Pronin A, Pham D, An W, Dvoriantchikova G, Reshetnikova G, Qiao J, Kozhekbaeva Z, Reiser AE, Slepak VZ, Shestopalov VI
Frontiers in molecular neuroscience 2019;12:36
Frontiers in molecular neuroscience 2019;12:36
E3 ubiquitin ligase Nedd4 is a key negative regulator for non-canonical inflammasome activation.
Liu Q, Zhang S, Sun Z, Guo X, Zhou H
Cell death and differentiation 2019 Nov;26(11):2386-2399
Cell death and differentiation 2019 Nov;26(11):2386-2399
Gasdermin D in peripheral myeloid cells drives neuroinflammation in experimental autoimmune encephalomyelitis.
Li S, Wu Y, Yang D, Wu C, Ma C, Liu X, Moynagh PN, Wang B, Hu G, Yang S
The Journal of experimental medicine 2019 Nov 4;216(11):2562-2581
The Journal of experimental medicine 2019 Nov 4;216(11):2562-2581
IRF2 transcriptionally induces GSDMD expression for pyroptosis.
Kayagaki N, Lee BL, Stowe IB, Kornfeld OS, O'Rourke K, Mirrashidi KM, Haley B, Watanabe C, Roose-Girma M, Modrusan Z, Kummerfeld S, Reja R, Zhang Y, Cho V, Andrews TD, Morris LX, Goodnow CC, Bertram EM, Dixit VM
Science signaling 2019 May 21;12(582)
Science signaling 2019 May 21;12(582)
Entamoeba histolytica-induced IL-1β secretion is dependent on caspase-4 and gasdermin D.
Quach J, Moreau F, Sandall C, Chadee K
Mucosal immunology 2019 Mar;12(2):323-339
Mucosal immunology 2019 Mar;12(2):323-339
Macrophage β2-Integrins Regulate IL-22 by ILC3s and Protect from Lethal Citrobacter rodentium-Induced Colitis.
Wang B, Lim JH, Kajikawa T, Li X, Vallance BA, Moutsopoulos NM, Chavakis T, Hajishengallis G
Cell reports 2019 Feb 5;26(6):1614-1626.e5
Cell reports 2019 Feb 5;26(6):1614-1626.e5
Trimetazidine Attenuates Cardiac Dysfunction in Endotoxemia and Sepsis by Promoting Neutrophil Migration.
Chen J, Wang B, Lai J, Braunstein Z, He M, Ruan G, Yin Z, Wang J, Cianflone K, Ning Q, Chen C, Wang DW
Frontiers in immunology 2018;9:2015
Frontiers in immunology 2018;9:2015
Guanylate binding proteins facilitate caspase-11-dependent pyroptosis in response to type 3 secretion system-negative Pseudomonas aeruginosa.
Balakrishnan A, Karki R, Berwin B, Yamamoto M, Kanneganti TD
Cell death discovery 2018;4:3
Cell death discovery 2018;4:3
Copper Regulates the Canonical NLRP3 Inflammasome.
Deigendesch N, Zychlinsky A, Meissner F
Journal of immunology (Baltimore, Md. : 1950) 2018 Mar 1;200(5):1607-1617
Journal of immunology (Baltimore, Md. : 1950) 2018 Mar 1;200(5):1607-1617
cGAS drives noncanonical-inflammasome activation in age-related macular degeneration.
Kerur N, Fukuda S, Banerjee D, Kim Y, Fu D, Apicella I, Varshney A, Yasuma R, Fowler BJ, Baghdasaryan E, Marion KM, Huang X, Yasuma T, Hirano Y, Serbulea V, Ambati M, Ambati VL, Kajiwara Y, Ambati K, Hirahara S, Bastos-Carvalho A, Ogura Y, Terasaki H, Oshika T, Kim KB, Hinton DR, Leitinger N, Cambier JC, Buxbaum JD, Kenney MC, Jazwinski SM, Nagai H, Hara I, West AP, Fitzgerald KA, Sadda SR, Gelfand BD, Ambati J
Nature medicine 2018 Jan;24(1):50-61
Nature medicine 2018 Jan;24(1):50-61
Caspase-11-mediated endothelial pyroptosis underlies endotoxemia-induced lung injury.
Cheng KT, Xiong S, Ye Z, Hong Z, Di A, Tsang KM, Gao X, An S, Mittal M, Vogel SM, Miao EA, Rehman J, Malik AB
The Journal of clinical investigation 2017 Nov 1;127(11):4124-4135
The Journal of clinical investigation 2017 Nov 1;127(11):4124-4135
Lipopolysaccharide-primed heterotolerant dendritic cells suppress experimental autoimmune uveoretinitis by multiple mechanisms.
Klaska IP, Muckersie E, Martin-Granados C, Christofi M, Forrester JV
Immunology 2017 Mar;150(3):364-377
Immunology 2017 Mar;150(3):364-377
Differential roles of caspase-1 and caspase-11 in infection and inflammation.
Man SM, Karki R, Briard B, Burton A, Gingras S, Pelletier S, Kanneganti TD
Scientific reports 2017 Mar 27;7:45126
Scientific reports 2017 Mar 27;7:45126
Differential Sensitivity of Target Genes to Translational Repression by miR-17~92.
Jin HY, Oda H, Chen P, Yang C, Zhou X, Kang SG, Valentine E, Kefauver JM, Liao L, Zhang Y, Gonzalez-Martin A, Shepherd J, Morgan GJ, Mondala TS, Head SR, Kim PH, Xiao N, Fu G, Liu WH, Han J, Williamson JR, Xiao C
PLoS genetics 2017 Feb;13(2):e1006623
PLoS genetics 2017 Feb;13(2):e1006623
Obesity-associated NLRC4 inflammasome activation drives breast cancer progression.
Kolb R, Phan L, Borcherding N, Liu Y, Yuan F, Janowski AM, Xie Q, Markan KR, Li W, Potthoff MJ, Fuentes-Mattei E, Ellies LG, Knudson CM, Lee MH, Yeung SJ, Cassel SL, Sutterwala FS, Zhang W
Nature communications 2016 Oct 6;7:13007
Nature communications 2016 Oct 6;7:13007
IRGB10 Liberates Bacterial Ligands for Sensing by the AIM2 and Caspase-11-NLRP3 Inflammasomes.
Man SM, Karki R, Sasai M, Place DE, Kesavardhana S, Temirov J, Frase S, Zhu Q, Malireddi RKS, Kuriakose T, Peters JL, Neale G, Brown SA, Yamamoto M, Kanneganti TD
Cell 2016 Oct 6;167(2):382-396.e17
Cell 2016 Oct 6;167(2):382-396.e17
NLRP3 regulates a non-canonical platform for caspase-8 activation during epithelial cell apoptosis.
Chung H, Vilaysane A, Lau A, Stahl M, Morampudi V, Bondzi-Simpson A, Platnich JM, Bracey NA, French MC, Beck PL, Chun J, Vallance BA, Muruve DA
Cell death and differentiation 2016 Aug;23(8):1331-46
Cell death and differentiation 2016 Aug;23(8):1331-46
SESN2/sestrin2 suppresses sepsis by inducing mitophagy and inhibiting NLRP3 activation in macrophages.
Kim MJ, Bae SH, Ryu JC, Kwon Y, Oh JH, Kwon J, Moon JS, Kim K, Miyawaki A, Lee MG, Shin J, Kim YS, Kim CH, Ryter SW, Choi AM, Rhee SG, Ryu JH, Yoon JH
Autophagy 2016 Aug 2;12(8):1272-91
Autophagy 2016 Aug 2;12(8):1272-91
Canonical Inflammasomes Drive IFN-γ to Prime Caspase-11 in Defense against a Cytosol-Invasive Bacterium.
Aachoui Y, Kajiwara Y, Leaf IA, Mao D, Ting JP, Coers J, Aderem A, Buxbaum JD, Miao EA
Cell host & microbe 2015 Sep 9;18(3):320-32
Cell host & microbe 2015 Sep 9;18(3):320-32
Caspase-11 cleaves gasdermin D for non-canonical inflammasome signalling.
Kayagaki N, Stowe IB, Lee BL, O'Rourke K, Anderson K, Warming S, Cuellar T, Haley B, Roose-Girma M, Phung QT, Liu PS, Lill JR, Li H, Wu J, Kummerfeld S, Zhang J, Lee WP, Snipas SJ, Salvesen GS, Morris LX, Fitzgerald L, Zhang Y, Bertram EM, Goodnow CC, Dixit VM
Nature 2015 Oct 29;526(7575):666-71
Nature 2015 Oct 29;526(7575):666-71
Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death.
Shi J, Zhao Y, Wang K, Shi X, Wang Y, Huang H, Zhuang Y, Cai T, Wang F, Shao F
Nature 2015 Oct 29;526(7575):660-5
Nature 2015 Oct 29;526(7575):660-5
The ketone metabolite β-hydroxybutyrate blocks NLRP3 inflammasome-mediated inflammatory disease.
Youm YH, Nguyen KY, Grant RW, Goldberg EL, Bodogai M, Kim D, D'Agostino D, Planavsky N, Lupfer C, Kanneganti TD, Kang S, Horvath TL, Fahmy TM, Crawford PA, Biragyn A, Alnemri E, Dixit VD
Nature medicine 2015 Mar;21(3):263-9
Nature medicine 2015 Mar;21(3):263-9
Escherichia coli α-hemolysin counteracts the anti-virulence innate immune response triggered by the Rho GTPase activating toxin CNF1 during bacteremia.
Diabate M, Munro P, Garcia E, Jacquel A, Michel G, Obba S, Goncalves D, Luci C, Marchetti S, Demon D, Degos C, Bechah Y, Mege JL, Lamkanfi M, Auberger P, Gorvel JP, Stuart LM, Landraud L, Lemichez E, Boyer L
PLoS pathogens 2015 Mar;11(3):e1004732
PLoS pathogens 2015 Mar;11(3):e1004732
Molecular mechanisms of cell death: central implication of ATP synthase in mitochondrial permeability transition.
Bonora M, Wieckowsk MR, Chinopoulos C, Kepp O, Kroemer G, Galluzzi L, Pinton P
Oncogene 2015 Mar 19;34(12):1608
Oncogene 2015 Mar 19;34(12):1608
Activation of the NLRP1b inflammasome independently of ASC-mediated caspase-1 autoproteolysis and speck formation.
Van Opdenbosch N, Gurung P, Vande Walle L, Fossoul A, Kanneganti TD, Lamkanfi M
Nature communications 2014;5:3209
Nature communications 2014;5:3209
Caspase-11 is expressed in the colonic mucosa and protects against dextran sodium sulfate-induced colitis.
Demon D, Kuchmiy A, Fossoul A, Zhu Q, Kanneganti TD, Lamkanfi M
Mucosal immunology 2014 Nov;7(6):1480-91
Mucosal immunology 2014 Nov;7(6):1480-91
Triggering receptor expressed on myeloid cells 1 (TREM-1)-mediated Bcl-2 induction prolongs macrophage survival.
Yuan Z, Syed MA, Panchal D, Joo M, Colonna M, Brantly M, Sadikot RT
The Journal of biological chemistry 2014 May 23;289(21):15118-29
The Journal of biological chemistry 2014 May 23;289(21):15118-29
FADD and caspase-8 mediate priming and activation of the canonical and noncanonical Nlrp3 inflammasomes.
Gurung P, Anand PK, Malireddi RK, Vande Walle L, Van Opdenbosch N, Dillon CP, Weinlich R, Green DR, Lamkanfi M, Kanneganti TD
Journal of immunology (Baltimore, Md. : 1950) 2014 Feb 15;192(4):1835-46
Journal of immunology (Baltimore, Md. : 1950) 2014 Feb 15;192(4):1835-46
Caspase-11 stimulates rapid flagellin-independent pyroptosis in response to Legionella pneumophila.
Case CL, Kohler LJ, Lima JB, Strowig T, de Zoete MR, Flavell RA, Zamboni DS, Roy CR
Proceedings of the National Academy of Sciences of the United States of America 2013 Jan 29;110(5):1851-6
Proceedings of the National Academy of Sciences of the United States of America 2013 Jan 29;110(5):1851-6
MicroRNA-17~92 plays a causative role in lymphomagenesis by coordinating multiple oncogenic pathways.
Jin HY, Oda H, Lai M, Skalsky RL, Bethel K, Shepherd J, Kang SG, Liu WH, Sabouri-Ghomi M, Cullen BR, Rajewsky K, Xiao C
The EMBO journal 2013 Aug 28;32(17):2377-91
The EMBO journal 2013 Aug 28;32(17):2377-91
Microglial cathepsin B contributes to the initiation of peripheral inflammation-induced chronic pain.
Sun L, Wu Z, Hayashi Y, Peters C, Tsuda M, Inoue K, Nakanishi H
The Journal of neuroscience : the official journal of the Society for Neuroscience 2012 Aug 15;32(33):11330-42
The Journal of neuroscience : the official journal of the Society for Neuroscience 2012 Aug 15;32(33):11330-42
An inactivating caspase 11 passenger mutation originating from the 129 murine strain in mice targeted for c-IAP1.
Kenneth NS, Younger JM, Hughes ED, Marcotte D, Barker PA, Saunders TL, Duckett CS
The Biochemical journal 2012 Apr 15;443(2):355-9
The Biochemical journal 2012 Apr 15;443(2):355-9
Non-canonical inflammasome activation targets caspase-11.
Kayagaki N, Warming S, Lamkanfi M, Vande Walle L, Louie S, Dong J, Newton K, Qu Y, Liu J, Heldens S, Zhang J, Lee WP, Roose-Girma M, Dixit VM
Nature 2011 Oct 16;479(7371):117-21
Nature 2011 Oct 16;479(7371):117-21
Distinct downstream pathways of caspase-11 in regulating apoptosis and cytokine maturation during septic shock response.
Kang SJ, Wang S, Kuida K, Yuan J
Cell death and differentiation 2002 Oct;9(10):1115-25
Cell death and differentiation 2002 Oct;9(10):1115-25
Dual role of caspase-11 in mediating activation of caspase-1 and caspase-3 under pathological conditions.
Kang SJ, Wang S, Hara H, Peterson EP, Namura S, Amin-Hanjani S, Huang Z, Srinivasan A, Tomaselli KJ, Thornberry NA, Moskowitz MA, Yuan J
The Journal of cell biology 2000 May 1;149(3):613-22
The Journal of cell biology 2000 May 1;149(3):613-22
Dual role of caspase-11 in mediating activation of caspase-1 and caspase-3 under pathological conditions.
Kang SJ, Wang S, Hara H, Peterson EP, Namura S, Amin-Hanjani S, Huang Z, Srinivasan A, Tomaselli KJ, Thornberry NA, Moskowitz MA, Yuan J
The Journal of cell biology 2000 May 1;149(3):613-22
The Journal of cell biology 2000 May 1;149(3):613-22
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Caspase 11 Antibody (17D9) [NB120-10454] - Mouse B cell. Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Caspase-11 Antibody (17D9) [NB120-10454] - Mouse liver extract was separated on a 4-15% gel and transfered to PVDF membrane. The membrane was probed with anti-caspase 11 antibody and detected with an anti-mouse HRP secondary antibody. Both procaspase 11 and fully cleaved caspase 11 are detected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Caspase-11 Antibody (17D9) [NB120-10454] - Caspase 11 Antibody (17D9) [NB120-10454] - analysis of Caspase 11 in LPS stimulated bone marrow derived macrophages using anti-Caspase 11 antibody. Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Caspase-11 Antibody (17D9) [NB120-10454] - Analysis of Caspase-11 in THP-1 cell lysates that were untreated (left lane) or treated (right lane) with 100 ng/ml LPS. Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Caspase-11 Antibody (17D9) [NB120-10454] - Casp11fl/fl and Casp11EC-/- mice (n = 3) were challenged with LPS (40 mg/kg i.p.) for 6 hours. Endothelial Cells were isolated from lungs, and western blot analysis was performed for pro-IL-1Beta, mature IL-1Beta, and caspase-1 cleavage. This image is from PMID: 28990935.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Caspase-11 Antibody (17D9) [NB120-10454] - IHC-P analysis of a formalin fixed paraffin embedded tissue section of mouse lung using Caspase 11 antibody (clone 17D9) at 1:100 diluton. The antibody generated cytoplasmic signal with more intense staining in the bronchiolar epithelium while the staining signal in alveolar cells was relatively low.
