A02941-2

antibody from Boster Biological Technology

Targeting: CASP5 ICE(rel)III

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Antibody Data

Product number

A02941-2

Provider

Boster Biological Technology

Product name

Anti-Caspase 4 Picoband antibody

Antibody type

Polyclonal

Description

Rabbit IgG polyclonal antibody for Caspase 4 detection. Tested with WB, Direct ELISA in Human;Mouse;Rat.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100ug/vial +free secondary

Storage

At -20 degrees C for one year. After reconstitution, at 4 degrees C for one month. It can also be aliquotted and stored frozen at -20 degrees C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of Caspase 4 using anti-Caspase 4 antibody (A02941-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse liver tissue lysates,Lane 2: mouse testis tissue lysates,Lane 3: mouse thymus tissue lysates,Lane 4: mouse lung tissue lysates,Lane 5: mouse HEPA1-6 whole cell lysates, Lane 6: mouse NIH3T3 whole cell lysates, Lane 7: mouse SP20 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase 4 antigen affinity purified polyclonal antibody (Catalog # A02941-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase 4 at approximately 43KD. The expected band size for Caspase 4 is at 43KD.

Additional image