PA5-39139

antibody from Invitrogen Antibodies

Targeting: POLR1B FLJ10816, FLJ21921, RPA135, RPA2, Rpo1-2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-39139

Provider

Invitrogen Antibodies

Product name

Anti-POLR1B Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RNA Polymerase I RPA2 in extracts from Jurkat and NIH-3T3 cells using a RNA Polymerase I RPA2 polyclonal antibody (Product # PA5-39139).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of HeLa (Lane 1), MCF7 (Lane 2), K562 (Lane 3), HT-29 (Lane 4), PC-3 (Lane 5), NTERA-2 (Lane 6) and HepG2 (Lane 7). The blot was probed with Anti-POLR1B Polyclonal Antibody (Product # PA5-39139, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 104 kDa band corresponding to POLR1B was observed in all cell lines tested.