MA5-25047

antibody from Invitrogen Antibodies

Targeting: PSMC3 TBP-1, TBP1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-25047

Provider

Invitrogen Antibodies

Product name

PSMC3 Monoclonal Antibody (OTI1C10)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human, Rat, Canine

Host

Mouse

Isotype

IgG

Antibody clone number

OTI1C10

Vial size

100 µL

Concentration

0.81 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PSMC3 in HepG2, HeLa, HT29, A549, COS7, Jurkat, MDCK, PC12, MCF7 cells using 35 µg per lane. Samples were probed with PSMC3 (Product # MA5-25047) monoclonal antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of PSMC3 in COS7 cells. Cells were transfected with a plasmid overexpressing PSMC3 and probed with a PSMC3 monoclonal antibody (Product # MA5-25047).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of PSMC3 in COS7 cells. Cells were transfected with a plasmid overexpressing PSMC3 and probed with a PSMC3 monoclonal antibody (Product # MA5-25047).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry was performed on paraffin-embedded kidney tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a PSMC3 monoclonal antibody (Product # MA5-25047) at a dilution of 1:50.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of ovary tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a PSMC3 monoclonal antibody (Product # MA5-25047) at a dilution of 1:50.