600-401-B24

antibody from Rockland Immunochemicals, Inc.

Targeting: SAE1 AOS1, FLJ3091, Sua1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

600-401-B24

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#600-401-B24, RRID:AB_2182937

Product name

Anti-SUMO Activating Enzyme E1 (SAE1) pS185 (RABBIT) Antibody - 600-401-B24

Antibody type

Polyclonal

Vial size

100 µl

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western blot using Rockland's Rabbit anti-SAE1 pS185 antibody shows detection of phosphorylated SAE1. Left lane (-) contains 20 µg human HeLa whole cell protein. Right lane (+) contains 20 µg human HeLa whole cell protein from cells pre-treated with phosphatase inhibitor cocktail to prevent dephos-phorylation of the target. Proteins were separated on a 10% SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% milk-TBST 1 hr at room temperature, the membrane was probed with the primary antibody diluted to 1:1,000 at room temperature for 3 hr followed by washes and reaction with HRP-conjugated secondary and ECL imaging. Personal communication, Xin-Hua Feng, Baylor College of Medicine, Houstin, TX

Validation comment

Western Blot