Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [6]
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Validation data
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- Product number
- PA1-32702 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CHRM2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA1-32702 detects CHRM2 from human samples.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 25 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-CHRM2 Polyclonal Antibody (Product # PA1-32702) and a 52 kDa band corresponding to CHRM2 was observed in Mouse Heart, Rat Heart, Cardiomyocytes but not in Mouse Brain, Mouse Kidney , Rat Kidney and iPSC. An uncharacterized band of ~110 kDa was also observed in iPSC and Cardiomyocytes. Tissue extracts (30 µg lysate) of Mouse Heart (Lane 1), Mouse Brain (Lane 2), Mouse Kidney (Lane 3), Rat Heart (Lane 4), Rat Kidney (Lane 5), whole cell extracts (30 µg lysate) of iPSC (Lane 6), and iPSC differentiated to Cardiomyocytes (Lane 7) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of CHRM2 in human brain tissue (neurons and glia) using CHRM2 Polyclonal Antibody (Product # PA1-32702). Antigen retrival : Heat-induced antigen retrieval.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical (Paraffin) analysis of brain, hypothalamus tissue using (Product # PA1-32702) CHRM2 Polyclonal Antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical (Paraffin) analysis of brain, parkinsons disease neuron lewy bodybrain, parkinsons disease neuron lewy body tissue using (Product # PA1-32702) CHRM2 Polyclonal Antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical (Paraffin) analysis of human brain, glioblastoma tissue using (Product # PA1-32702) CHRM2 Polyclonal Antibody. Antigen retrieval: Heat-induced antigen retrieval.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical (Paraffin) analysis of human brain, neurons and glia tissue using (Product # PA1-32702) CHRM2 Polyclonal Antibody. Antigen retrieval: Heat-induced antigen retrieval.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical (Paraffin) analysis of human skin, melanoma tissue using (Product # PA1-32702) CHRM2 Polyclonal Antibody. Antigen retrieval: Heat-induced antigen retrieval.