GTX31063

antibody from GeneTex

Targeting: CHRM2

Western blot (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX31063

Provider

GeneTex

Proper citation

GeneTex Cat#GTX31063, RRID:AB_10732336

Product name

mAChR M2 antibody [31-1D1]

Antibody type

Monoclonal

Reactivity

Human, Mouse, Rat, Porcine

Host

Mouse

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor 2 Antibody (31-1D1) [GTX31063] - Immunohistochemistry was performed on normal biopsies of deparaffinized human kidney tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a Mouse Monoclonal Antibody recognizing Muscarinic Acetylcholine Receptor M2 or without primary antibody (negative control) overnight at 4C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Tissues were counterstained with hematoxylin and prepped for mounting