Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX31063 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX31063, RRID:AB_10732336
- Product name
- mAChR M2 antibody [31-1D1]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat, Porcine
- Host
- Mouse
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor 2 Antibody (31-1D1) [GTX31063] - Immunohistochemistry was performed on normal biopsies of deparaffinized human kidney tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a Mouse Monoclonal Antibody recognizing Muscarinic Acetylcholine Receptor M2 or without primary antibody (negative control) overnight at 4C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Tissues were counterstained with hematoxylin and prepped for mounting