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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- MA3-044 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CHRM2 Monoclonal Antibody (31-1D1)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA3-044 detects muscarinic 2 acetylcholine receptor (m2AChR) from human, mouse, pig and rat tissues. This antibody does not detect mAChR from chicken tissues. MA3-044 is specific for the m2 mAChR subtype. MA3-044 has been successfully used in Western blot and immunoprecipitation protocols. MA3-044 is an ~52kDa protein; however, by Western blot this antibody may detect 60-80 kDa protein(s) (depending on the degree of glycosylation) representing the m2AChR from pig heart. The MA3-044 antigen is purified porcine cardiac m2 AChR.
- Reactivity
- Human, Mouse, Rat, Porcine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 31-1D1
- Vial size
- 250 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
Submitted references Enhanced Contractive Tension and Upregulated Muscarinic Receptor 2/3 in Colorectum Contribute to Constipation in 6-Hydroxydopamine-Induced Parkinson's Disease Rats.
Smooth muscle adaptation and recovery of contractility after massive small bowel resection in rats.
Cleavage-resistant fusion proteins of the M(2) muscarinic receptor and Gα(i1). Homotropic and heterotropic effects in the binding of ligands.
Cholesterol as a determinant of cooperativity in the M2 muscarinic cholinergic receptor.
Cooperativity manifest in the binding properties of purified cardiac muscarinic receptors.
Muscarinic acetylcholine receptor subtypes mediating urinary bladder contractility and coupling to GTP binding proteins.
Isolation and characterization of monoclonal antibodies specific for the cardiac muscarinic acetylcholine receptor.
Zhang XL, Zhang XH, Yu X, Zheng LF, Feng XY, Liu CZ, Quan ZS, Zhang Y, Zhu JX
Frontiers in aging neuroscience 2021;13:770841
Frontiers in aging neuroscience 2021;13:770841
Smooth muscle adaptation and recovery of contractility after massive small bowel resection in rats.
Chen J, Wen J, Cai W
Experimental biology and medicine (Maywood, N.J.) 2012 May;237(5):578-84
Experimental biology and medicine (Maywood, N.J.) 2012 May;237(5):578-84
Cleavage-resistant fusion proteins of the M(2) muscarinic receptor and Gα(i1). Homotropic and heterotropic effects in the binding of ligands.
Ma AW, Dong JY, Ma D, Wells JW
Biochimica et biophysica acta 2011 Jun;1810(6):592-602
Biochimica et biophysica acta 2011 Jun;1810(6):592-602
Cholesterol as a determinant of cooperativity in the M2 muscarinic cholinergic receptor.
Colozo AT, Park PS, Sum CS, Pisterzi LF, Wells JW
Biochemical pharmacology 2007 Jul 15;74(2):236-55
Biochemical pharmacology 2007 Jul 15;74(2):236-55
Cooperativity manifest in the binding properties of purified cardiac muscarinic receptors.
Wreggett KA, Wells JW
The Journal of biological chemistry 1995 Sep 22;270(38):22488-99
The Journal of biological chemistry 1995 Sep 22;270(38):22488-99
Muscarinic acetylcholine receptor subtypes mediating urinary bladder contractility and coupling to GTP binding proteins.
Wang P, Luthin GR, Ruggieri MR
The Journal of pharmacology and experimental therapeutics 1995 May;273(2):959-66
The Journal of pharmacology and experimental therapeutics 1995 May;273(2):959-66
Isolation and characterization of monoclonal antibodies specific for the cardiac muscarinic acetylcholine receptor.
Luetje CW, Brumwell C, Norman MG, Peterson GL, Schimerlik MI, Nathanson NM
Biochemistry 1987 Nov 3;26(22):6892-6
Biochemistry 1987 Nov 3;26(22):6892-6
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Muscarinic Acetylcholine Receptor M2 was done on 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled Muscarinic Acetylcholine Receptor M2 (31-1D1) Mouse Monoclonal Antibody (Product # MA3-044) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on normal biopsies of deparaffinized human kidney tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a Mouse Monoclonal Antibody recognizing Muscarinic Acetylcholine Receptor M2 (Product # MA3-044) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
