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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- RQ8594 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- PAWR Antibody / PRKC apoptosis WT1 regulator protein / Par4
- Antibody type
- Polyclonal
- Description
- This highly specific PAWR antibody is suitable for use in Western blot/Immunofluorescence/Flow cytometry/ELISA applications with human samples.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the PAWR antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) SW620, 2) SiHa, 3) RT4 and 4) Caco-2 cell lysate with PAWR antibody. Predicted molecular weight ~35 kDa, commonly observed at 35-43 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human HeLa cells with PAWR antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of fixed and permeabilized human Caco-2 cells with PAWR antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PAWR antibody.
