Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- ABIN499175 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Ligase I, DNA, ATP-Dependent (LIG1) (Internal Region) antibody
- Antibody type
- Polyclonal
- Antigen
- Peptide from the internal region (near C-Terminus) of the protein sequence according to NP_000225.1
- Description
- Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
- Reactivity
- Human, Rat
- Host
- Goat
- Antigen sequence
C-RVREDKQPEQATTS
- Epitope
- Internal Region
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 2 - 8°C up to one week or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Cell cycle-dependent phosphorylation of human DNA ligase I at the cyclin-dependent kinase sites.
Ferrari G, Rossi R, Arosio D, Vindigni A, Biamonti G, Montecucco A
The Journal of biological chemistry 2003 Sep 26;278(39):37761-7
The Journal of biological chemistry 2003 Sep 26;278(39):37761-7
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- AP26017PU-N (0.1μg/ml) staining of HeLa nuclear lysate (35μg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.