Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- PA5-27820 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DNA Ligase I Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji. Predicted reactivity: Mouse (92%), Rat (92%), Bovine (89%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references PCNA dependent cellular activities tolerate dramatic perturbations in PCNA client interactions.
Wilson RH, Biasutto AJ, Wang L, Fischer R, Baple EL, Crosby AH, Mancini EJ, Green CM
DNA repair 2017 Feb;50:22-35
DNA repair 2017 Feb;50:22-35
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using DNA Ligase I Polyclonal Antibody (Product # PA5-27820). Sample (30 µg of whole cell lysate). Lane A: A431. Lane B: H1299 . 7.5% SDS PAGE. DNA Ligase I Polyclonal Antibody (Product # PA5-27820) diluted at 1:1,000.