LS-C148305

antibody from LSBio

Targeting: CARD8 CARDINAL, Dakar, KIAA0955, NDPP, TUCAN

Western blot (Enhanced validation)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C148305

Provider

LSBio

Product name

CARD8 / Cardinal / TUCAN Antibody LS-C148305

Antibody type

Polyclonal

Description

Antiserum

Reactivity

Human

Host

Rabbit

Storage

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot of TUCAN/CARD8 in tumor cell lines using Polyclonal Antibody to TUCAN/CARD8 at 1:2000. Cell lysates were normalized for total protein content.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Formalin-fixed, paraffin-embedded tumor/normal adjacent tissue cores from a human colontissue microarray stained for TUCAN/CARD8 expression at 1:2000 using Polyclonal Antibody to TUCAN/CARD8. A and B are tumor tissue cores. A1 and B1 are the matched normal adjacent cores from A and B, respectively. Hematoxylin-Eos in counterstain.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Formalin-fixed, paraffin-embedded tissue human colon microarray stained for TUCAN/CARD8 expression at 1:2000 using Polyclonal Antibody to TUCAN/CARD8. A-A2, successively higher magnifications of the microarray. A, low magnification over view. A1, differential TUCAN/CARD8 staining between tissue cores is observed. A2, Areas of intense TUCAN/CARD8 staining in a colon carcinoma tissue core. Hematoxylin Eosin counterstain.