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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-22004 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LITAF Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, HepG2.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), Hep G2 treated with LPS (200ng/ml for 3 h) (Lane 2) and HeLa (Lane 3). The blot was probed with Anti-LITAF Polyclonal Antibody (Product # PA5-22004, 1 µg/ml) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 24 kDa band corresponding to LITAF was observed in the cell lines tested and was enhanced upon LPS treatment in Hep G2 cell line.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using LITAF Polyclonal Antibody (Product # PA5-22004). Sample (30 µg of whole cell lysate). A: Hep G2. 12% SDS PAGE. LITAF Polyclonal Antibody (Product # PA5-22004) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of LITAF was performed using 70% confluent log phase THP-1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with LITAF Polyclonal Antibody (Product # PA5-22004) at 5 µg/mL concentration in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nucleus and cytoplasm localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- LITAF Polyclonal Antibody detects LITAF protein at cytoplasm on human placenta by immunohistochemical analysis. Sample: Paraffin-embedded placenta. LITAF Polyclonal Antibody (Product # PA5-22004) dilution: 1:100. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
