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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- NBP1-50033 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-50033, RRID:AB_10012223
- Product name
- Rabbit Polyclonal IBTK Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Function of inhibitor of Bruton's tyrosine kinase isoform α (IBTKα) in nonalcoholic steatohepatitis links autophagy and the unfolded protein response.
Willy JA, Young SK, Mosley AL, Gawrieh S, Stevens JL, Masuoka HC, Wek RC
The Journal of biological chemistry 2017 Aug 25;292(34):14050-14065
The Journal of biological chemistry 2017 Aug 25;292(34):14050-14065
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: IBTK Antibody [NBP1-50033] - Detection of Human IBtk by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-IBtk antibody used for WB at 0.1 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate. IBtk was also immunoprecipitated by rabbit anti-IBtk antibody BL10880, which recognizes an upstream epitope. For blotting immunoprecipitated IBtk, the ReliaBLOT Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).
