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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- TA503562 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA503562, RRID:AB_11125669
- Product name
- EIF2B3 mouse monoclonal antibody, clone OTI1H3 (formerly 1H3)
- Antibody type
- Monoclonal
- Description
- EIF2B3 mouse monoclonal antibody, clone OTI1H3 (formerly 1H3)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- EIF2B3
- Isotype
- IgG
- Antibody clone number
- OTI1H3
- Vial size
- 100 µl
- Concentration
- 0.66 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EIF2B3 (RC223570, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF2B3.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 5 different cell lines by using anti-EIF2B3 monoclonal antibody.(1:200)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-EIF2B3 mouse monoclonal antibody (TA503562) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY EIF2B3(RC223570).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-EIF2B3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503562)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-EIF2B3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503562)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-EIF2B3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503562)
- Validation comment
- IHC
