Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [6]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- 27155-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Caspase 7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MADEQGCIE EQGVEDSANE DSVDAKPDRS SFVPSLFSKK KKNVTMRSIK TTRDRVPTYQ YNMNFEKLGK CIIINNKNFD KVTGMGVRNG TDKDAEALFK C (1-100 aa encoded by BC015799)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.4 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WB result of Caspase 7 antibody (27155-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caspase 7 transfected HEK-293 cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 27155-1-AP (Caspase 7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- LNCaP cells were subjected to SDS PAGE followed by western blot with 27155-1-AP ( Caspase 7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- LNCaP cells were subjected to SDS PAGE followed by western blot with 27155-1-AP (Caspase 7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF-7 cells were subjected to SDS PAGE followed by western blot with 27155-1-AP (Caspase 7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse liver tissue were subjected to SDS PAGE followed by western blot with 27155-1-AP (Caspase 7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol ) fixed MCF-7 cells using 27155-1-AP (Caspase 7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-Caspase 7 (IP:27155-1-AP, 4ug; Detection:27155-1-AP 1:1000) with HEK-293 cells lysate 2600 ug.