MA1-41281

antibody from Invitrogen Antibodies

Targeting: CASP7 CMH-1, ICE-LAP3, MCH3

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-41281

Provider

Invitrogen Antibodies

Product name

Caspase 7 Monoclonal Antibody (25B881.1)

Antibody type

Monoclonal

Antigen

Other

Description

Suggested positive control: Jurkat, antigen standard for CASP7 (transient overexpression lysate), Jurkat whole cell lysate.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

25B881.1

Vial size

100 µg

Concentration

1.0 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Caspase-7 in A) human brain, B) mouse brain and C) rat brain lysate using Caspase 7 monoclonal antibody (Product # MA1-41281) at 1 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Caspase 7 in Jurkat cells . Samples were incubated in Caspase 7 monoclonal antibody (Product # MA1-41281 using a dilution of 1 µg/mL. Cells were treated with 2 µM staurosporine for different time periods. Caspase-7 activation is detected in s by the presence of Caspase-7 cleavage fragments. The antibody detected both pro (full-length) and active (cleaved) Caspase-7, depending on the treatment time points. A basal level of endogenous intermediate Caspase-7 can be see in untreated Jurkat cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Caspase 7 in A) human brain, B) mouse brain and C) rat brain lysate. Samples were incubated in Caspase 7 monoclonal antibody (Product # MA1-41281) using a dilution of 1 µg/mL.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Caspase 7 in Human tonsil. Samples were incubated with Caspase 7 monoclonal antibody (Product # MA1-41281) using a dilution of 5 µg/mL. Human tonsil tissue slide was used for this test. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.