PA5-29399

antibody from Invitrogen Antibodies

Targeting: PTTG1 EAP1, HPTTG, PTTG, securin, TUTR1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-29399

Provider

Invitrogen Antibodies

Product name

Securin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Recommended positive controls: IMR32. Predicted reactivity: Rhesus Monkey (98%), Chimpanzee (99%), Bovine (85%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Signatures of Multi-Omics Reveal Distinct Tumor Immune Microenvironment Contributing to Immunotherapy in Lung Adenocarcinoma.

Huang Z, Li B, Guo Y, Wu L, Kou F, Yang L
Frontiers in immunology 2021;12:723172

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Securin Polyclonal Antibody (Product # PA5-29399). Sample (30 µg of whole cell lysate). Lane A: IMR32. 12% SDS PAGE. Securin Polyclonal Antibody (Product # PA5-29399) diluted at 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Securin Polyclonal Antibody detects PTTG1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PTTG1 protein stained by Securin Polyclonal Antibody (Product # PA5-29399) diluted at 1:500. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Securin Polyclonal Antibody detects PTTG1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PTTG1 protein stained by Securin Polyclonal Antibody (Product # PA5-29399) diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Securin Polyclonal Antibody detects PTTG1 protein at on BT483 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded BT483 xenograft. Securin Polyclonal Antibody (Product # PA5-29399) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Securin Polyclonal Antibody immunoprecipitates Securin protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. Control with 2 µg of preimmune rabbit IgG B. Immunoprecipitation of Securin protein by 2 µg of Securin Polyclonal Antibody (Product # PA5-29399) 12% SDS-PAGE The immunoprecipitated Securin protein was detected by Securin Polyclonal Antibody (Product # PA5-29399) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4 Validation of the prognostic risk model in the LUAD cohort. (A) Kaplan-Meier curves for OS by risk subgroups in the GSE31210. (B) Time-dependent ROC curves measuring the prognostic value of the risk score at 3 and 5 years in the GSE31210. (C) Kaplan-Meier curves for OS by risk subgroups in the mRNA level from the Tianjin cohort. (D) Representative IHC images for staining of signatures in 180 samples of patients with LUAD (Olympus, x400 magnification, bar = 50 mum). (E) Heat map of PTTG1 , SLC2A1 , and FAM83A in the Tianjin cohort (upper figure). Distribution of risk score (middle figure), and OS (lower figure). (F) Kaplan-Meier curves for OS by risk subgroups in the Tianjin cohort. (G, H) Time-dependent ROC curves measuring the prognostic value of the risk score and stage at (G) 3 years and (H) 5 years in the Tianjin cohort. (I) Forest plot showing the independent prognostic factors for OS in the Tianjin cohort (multivariate Cox regression analysis). (J) Nomogram for predicting 1-, 3-, and 5-year overall survival in the Tianjin cohort. *p < 0.05 and ***p < 0.001.