Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-49709 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PDCD10 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The antibody detects endogenous level of total PDCD10 protein.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.6 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PDCD10 was performed by loading Lane1: Hela cell lysate (50µg), Lane2: Jurkat cell lysate (50µg), Lane3: 231 cell lysate (50µg) on to a 12% SDS-PAGE gel. Proteins were transferred to a membrane and the membrane was probed with a PDCD10 antibody (Product # PA5-49709) at a 1/200dilution for 10 seconds, followed by Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- (Left) Immunohistochemical analysis of PDCD10 in paraffin-embedded human tonsil tissue. Samples were probed using a PDCD10 polyclonal antibody (Product # PA5-49709) at dilution 1/20, (Right) treated with the fusion protein.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PDCD10 in paraffin-embedded human tonsil tissue using (left) PDCD10 Polyclonal Antibody (Product # PA5-49709) at a 1:20 dilution and (right) is treated with the fusion protein.