Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Flow cytometry [1]
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Validation data
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- Product number
- GTX17959 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX17959, RRID:AB_422995
- Product name
- MLL antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Kmt2a cooperates with menin to suppress tumorigenesis in mouse pancreatic islets.
Lin W, Francis JM, Li H, Gao X, Pedamallu CS, Ernst P, Meyerson M
Cancer biology & therapy 2016 Dec;17(12):1274-1281
Cancer biology & therapy 2016 Dec;17(12):1274-1281
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- "Western Blot: MLL-1 Antibody (GTX17959) - Detection of Human and Mouse MLL1 by Western Blot. Samples: Nuclear extract (50 mcg for WT and KO; 10 mcg for EF, EN and EC) from 293T cells that were transfected with MLL1 c-terminal 180 kDa fragment (EC), 293T cells transfected with N-terminally truncated MLL1 fragment (EN; N300-tr), 293T cells transfected with MLL1 N-terminal 300 kDa fragment (EF), MLL1 +/+ mouse embryonic stem cells (WT), and MLL1 -/- embryonic stem cells (KO). Antibody: GTX17959 used at the indicated concentrations Detection: One second exposure with chemiluminescence"
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- "Western Blot: KMT2A/MLL Antibody (GTX17959) - Nuclear extract (NE; 15 and 50 mcg) and whole cell lysate (WCL; 15 and 50 mcg) from HeLa cells. Antibodies: Affinity purified rabbit anti-MLL1 antibody."
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Flow Cytometry: MLL-1 Antibody (GTX17959) - Flow cytometric detection of MLL1. 1 million Jurkat cells were fixed, permeabilized, and stained with 3.0 mcg/ml anti-MLL1 GTX17959 in a 150 mcl reaction. Isotype control (black), anti- MLL1 (red).