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LearnNB600-256
antibody from Novus Biologicals
Targeting: KMT2A
ALL-1, CXXC7, HRX, HTRX1, MLL, MLL1A, TRX1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [3]
- Immunoprecipitation [1]
- Flow cytometry [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- NB600-256 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-256, RRID:AB_10002583
- Product name
- Rabbit Polyclonal KMT2A/MLL Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Epigenetic regulation of IL-12-dependent T cell proliferation.
Additional sex comb-like (ASXL) proteins 1 and 2 play opposite roles in adipogenesis via reciprocal regulation of peroxisome proliferator-activated receptor {gamma}.
Schaller M, Ito T, Allen RM, Kroetz D, Kittan N, Ptaschinski C, Cavassani K, Carson WF 4th, Godessart N, Grembecka J, Cierpicki T, Dou Y, Kunkel SL
Journal of leukocyte biology 2015 Oct;98(4):601-13
Journal of leukocyte biology 2015 Oct;98(4):601-13
Additional sex comb-like (ASXL) proteins 1 and 2 play opposite roles in adipogenesis via reciprocal regulation of peroxisome proliferator-activated receptor {gamma}.
Park UH, Yoon SK, Park T, Kim EJ, Um SJ
The Journal of biological chemistry 2011 Jan 14;286(2):1354-63
The Journal of biological chemistry 2011 Jan 14;286(2):1354-63
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: KMT2A/MLL Antibody [NB600-256] - Detection of the C-terminal 180 kDa Fragment of MLL1 by Western Blot and Immunoprecipitation. Samples: A. Nuclear extract from K562 cells (N; 50 mcg/lane), 293T cells transfected with full-length MLL1 (F; 15 mcg/lane) and 293T cells transfected with the C-terminal 180kDa fragment of MLL1 (C; 15 mcg/lane). B. Nuclear extract (1 mg) from K563 cells. Antibodies: Affinity purified rabbit anti-MLL1 antibody used at 0.1 mcg/ml (A) and 0.4 mcg/ml (B) for blotting. Immunoprecipitations were performed using NB 600-256, NB 600-257, and another MLL1 antibody at 1 mcg/mg lysate. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 1 minute (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: KMT2A/MLL Antibody [NB600-256] - Detection of Human MLL1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MLL1 antibody NB600-256 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: KMT2A/MLL Antibody [NB600-256] - Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MLL1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: KMT2A/MLL Antibody [NB600-256] - Detection of human MLL1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MLL1 antibody NB600-256 used for IP at 3 ug per reaction. MLL1 was also immunoprecipitated by another rabbit anti-MLL1 antibody. For blotting immunoprecipitated MLL1, NB600-256 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: KMT2A/MLL Antibody [NB600-256] - Flow cytometry testing of KMT2A/MLL antibody on murine WT and Mll1-/- CD4 T cells that were stimulated in-vitro with anti-CD3/ -CD28 antibodies. Image submitted by our verified customer (see Review section for additional details).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation: KMT2A/MLL Antibody [NB600-256] - A. 10 ug of was used to immunoprecipitate chromatin from K562 cells according to Ren et al (Genes Dev. 2002 16: 245-256).
