MA5-15380

antibody from Invitrogen Antibodies

Targeting: KMT2A ALL-1, CXXC7, HRX, HTRX1, MLL, MLL1A, TRX1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15380

Provider

Invitrogen Antibodies

Product name

HRX Monoclonal Antibody (10F8D7)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-15380 targets MLL in indirect ELISA, IHC and WB applications and shows reactivity with Human samples. The MA5-15380 immunogen is purified recombinant fragment of MLL (aa3751-3968) expressed in E. Coli.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

10F8D7

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of MLL using a MLL monoclonal antibody (Product # MA5-15380) against a truncated MLL recombinant protein (1) and truncated GFP-MLL (aa3714-3969) transfected COS-7 cell lysate (2).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of MLL using a MLL monoclonal antibody (Product # MA5-15380) against a truncated MLL recombinant protein (1) and truncated GFP-MLL (aa3714-3969) transfected COS-7 cell lysate (2).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of COS cells using LPP monoclonal antibody (Product # MA5-15379) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled using DY-554 phalloidin.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human lung cancer (left) and esophagus cancer (right), showing nuclear weak staining using MLL monoclonal antibody (Product # MA5-15380) followed with DAB staining