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LearnNB600-248
antibody from Novus Biologicals
Targeting: KMT2A
ALL-1, CXXC7, HRX, HTRX1, MLL, MLL1A, TRX1
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [26]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
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- Product number
- NB600-248 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-248, RRID:AB_2145479
- Product name
- Rabbit Polyclonal KMT2A/MLL Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references HOTTIP lncRNA Promotes Hematopoietic Stem Cell Self-Renewal Leading to AML-like Disease in Mice.
Mll-AF4 Confers Enhanced Self-Renewal and Lymphoid Potential during a Restricted Window in Development.
Pregnancy-induced noncoding RNA (PINC) associates with polycomb repressive complex 2 and regulates mammary epithelial differentiation.
Base-pair resolution DNA methylation sequencing reveals profoundly divergent epigenetic landscapes in acute myeloid leukemia.
Reduction of Hox gene expression by histone H1 depletion.
Mesenchymal stem/stromal cells induce the generation of novel IL-10-dependent regulatory dendritic cells by SOCS3 activation.
MLL fusion proteins preferentially regulate a subset of wild-type MLL target genes in the leukemic genome.
Pygo2 associates with MLL2 histone methyltransferase and GCN5 histone acetyltransferase complexes to augment Wnt target gene expression and breast cancer stem-like cell expansion.
Identification and characterization of a novel human PP1 phosphatase complex.
Gamma interferon-dependent transcriptional memory via relocalization of a gene locus to PML nuclear bodies.
DDB1-CUL4 and MLL1 mediate oncogene-induced p16INK4a activation.
Transcriptional activation of the mixed lineage leukemia-p27Kip1 pathway by a somatostatin analogue.
Long noncoding RNAs in mouse embryonic stem cell pluripotency and differentiation.
SWI/SNF mediates polycomb eviction and epigenetic reprogramming of the INK4b-ARF-INK4a locus.
Wdr82 is a C-terminal domain-binding protein that recruits the Setd1A Histone H3-Lys4 methyltransferase complex to transcription start sites of transcribed human genes.
Molecular regulation of H3K4 trimethylation by Wdr82, a component of human Set1/COMPASS.
Protein arginine-methyltransferase-dependent oncogenesis.
Prefrontal dysfunction in schizophrenia involves mixed-lineage leukemia 1-regulated histone methylation at GABAergic gene promoters.
Bimodal degradation of MLL by SCFSkp2 and APCCdc20 assures cell cycle execution: a critical regulatory circuit lost in leukemogenic MLL fusions.
The coactivator host cell factor-1 mediates Set1 and MLL1 H3K4 trimethylation at herpesvirus immediate early promoters for initiation of infection.
The APC tumor suppressor counteracts beta-catenin activation and H3K4 methylation at Wnt target genes.
Myc-binding-site recognition in the human genome is determined by chromatin context.
Genome-wide analysis of menin binding provides insights into MEN1 tumorigenesis.
Histone and DNA methylation defects at Hox genes in mice expressing a SET domain-truncated form of Mll.
Physical association and coordinate function of the H3 K4 methyltransferase MLL1 and the H4 K16 acetyltransferase MOF.
Menin and MLL cooperatively regulate expression of cyclin-dependent kinase inhibitors.
Luo H, Zhu G, Xu J, Lai Q, Yan B, Guo Y, Fung TK, Zeisig BB, Cui Y, Zha J, Cogle C, Wang F, Xu B, Yang FC, Li W, So CWE, Qiu Y, Xu M, Huang S
Cancer cell 2019 Dec 9;36(6):645-659.e8
Cancer cell 2019 Dec 9;36(6):645-659.e8
Mll-AF4 Confers Enhanced Self-Renewal and Lymphoid Potential during a Restricted Window in Development.
Barrett NA, Malouf C, Kapeni C, Bacon WA, Giotopoulos G, Jacobsen SEW, Huntly BJ, Ottersbach K
Cell reports 2016 Jul 26;16(4):1039-1054
Cell reports 2016 Jul 26;16(4):1039-1054
Pregnancy-induced noncoding RNA (PINC) associates with polycomb repressive complex 2 and regulates mammary epithelial differentiation.
Shore AN, Kabotyanski EB, Roarty K, Smith MA, Zhang Y, Creighton CJ, Dinger ME, Rosen JM
PLoS genetics 2012;8(7):e1002840
PLoS genetics 2012;8(7):e1002840
Base-pair resolution DNA methylation sequencing reveals profoundly divergent epigenetic landscapes in acute myeloid leukemia.
Akalin A, Garrett-Bakelman FE, Kormaksson M, Busuttil J, Zhang L, Khrebtukova I, Milne TA, Huang Y, Biswas D, Hess JL, Allis CD, Roeder RG, Valk PJ, Löwenberg B, Delwel R, Fernandez HF, Paietta E, Tallman MS, Schroth GP, Mason CE, Melnick A, Figueroa ME
PLoS genetics 2012;8(6):e1002781
PLoS genetics 2012;8(6):e1002781
Reduction of Hox gene expression by histone H1 depletion.
Zhang Y, Liu Z, Medrzycki M, Cao K, Fan Y
PloS one 2012;7(6):e38829
PloS one 2012;7(6):e38829
Mesenchymal stem/stromal cells induce the generation of novel IL-10-dependent regulatory dendritic cells by SOCS3 activation.
Liu X, Qu X, Chen Y, Liao L, Cheng K, Shao C, Zenke M, Keating A, Zhao RC
Journal of immunology (Baltimore, Md. : 1950) 2012 Aug 1;189(3):1182-92
Journal of immunology (Baltimore, Md. : 1950) 2012 Aug 1;189(3):1182-92
MLL fusion proteins preferentially regulate a subset of wild-type MLL target genes in the leukemic genome.
Wang QF, Wu G, Mi S, He F, Wu J, Dong J, Luo RT, Mattison R, Kaberlein JJ, Prabhakar S, Ji H, Thirman MJ
Blood 2011 Jun 23;117(25):6895-905
Blood 2011 Jun 23;117(25):6895-905
Pygo2 associates with MLL2 histone methyltransferase and GCN5 histone acetyltransferase complexes to augment Wnt target gene expression and breast cancer stem-like cell expansion.
Chen J, Luo Q, Yuan Y, Huang X, Cai W, Li C, Wei T, Zhang L, Yang M, Liu Q, Ye G, Dai X, Li B
Molecular and cellular biology 2010 Dec;30(24):5621-35
Molecular and cellular biology 2010 Dec;30(24):5621-35
Identification and characterization of a novel human PP1 phosphatase complex.
Lee JH, You J, Dobrota E, Skalnik DG
The Journal of biological chemistry 2010 Aug 6;285(32):24466-76
The Journal of biological chemistry 2010 Aug 6;285(32):24466-76
Gamma interferon-dependent transcriptional memory via relocalization of a gene locus to PML nuclear bodies.
Gialitakis M, Arampatzi P, Makatounakis T, Papamatheakis J
Molecular and cellular biology 2010 Apr;30(8):2046-56
Molecular and cellular biology 2010 Apr;30(8):2046-56
DDB1-CUL4 and MLL1 mediate oncogene-induced p16INK4a activation.
Kotake Y, Zeng Y, Xiong Y
Cancer research 2009 Mar 1;69(5):1809-14
Cancer research 2009 Mar 1;69(5):1809-14
Transcriptional activation of the mixed lineage leukemia-p27Kip1 pathway by a somatostatin analogue.
Horiguchi K, Yamada M, Satoh T, Hashimoto K, Hirato J, Tosaka M, Yamada S, Mori M
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Apr 15;15(8):2620-9
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Apr 15;15(8):2620-9
Long noncoding RNAs in mouse embryonic stem cell pluripotency and differentiation.
Dinger ME, Amaral PP, Mercer TR, Pang KC, Bruce SJ, Gardiner BB, Askarian-Amiri ME, Ru K, Soldà G, Simons C, Sunkin SM, Crowe ML, Grimmond SM, Perkins AC, Mattick JS
Genome research 2008 Sep;18(9):1433-45
Genome research 2008 Sep;18(9):1433-45
SWI/SNF mediates polycomb eviction and epigenetic reprogramming of the INK4b-ARF-INK4a locus.
Kia SK, Gorski MM, Giannakopoulos S, Verrijzer CP
Molecular and cellular biology 2008 May;28(10):3457-64
Molecular and cellular biology 2008 May;28(10):3457-64
Wdr82 is a C-terminal domain-binding protein that recruits the Setd1A Histone H3-Lys4 methyltransferase complex to transcription start sites of transcribed human genes.
Lee JH, Skalnik DG
Molecular and cellular biology 2008 Jan;28(2):609-18
Molecular and cellular biology 2008 Jan;28(2):609-18
Molecular regulation of H3K4 trimethylation by Wdr82, a component of human Set1/COMPASS.
Wu M, Wang PF, Lee JS, Martin-Brown S, Florens L, Washburn M, Shilatifard A
Molecular and cellular biology 2008 Dec;28(24):7337-44
Molecular and cellular biology 2008 Dec;28(24):7337-44
Protein arginine-methyltransferase-dependent oncogenesis.
Cheung N, Chan LC, Thompson A, Cleary ML, So CW
Nature cell biology 2007 Oct;9(10):1208-15
Nature cell biology 2007 Oct;9(10):1208-15
Prefrontal dysfunction in schizophrenia involves mixed-lineage leukemia 1-regulated histone methylation at GABAergic gene promoters.
Huang HS, Matevossian A, Whittle C, Kim SY, Schumacher A, Baker SP, Akbarian S
The Journal of neuroscience : the official journal of the Society for Neuroscience 2007 Oct 17;27(42):11254-62
The Journal of neuroscience : the official journal of the Society for Neuroscience 2007 Oct 17;27(42):11254-62
Bimodal degradation of MLL by SCFSkp2 and APCCdc20 assures cell cycle execution: a critical regulatory circuit lost in leukemogenic MLL fusions.
Liu H, Cheng EH, Hsieh JJ
Genes & development 2007 Oct 1;21(19):2385-98
Genes & development 2007 Oct 1;21(19):2385-98
The coactivator host cell factor-1 mediates Set1 and MLL1 H3K4 trimethylation at herpesvirus immediate early promoters for initiation of infection.
Narayanan A, Ruyechan WT, Kristie TM
Proceedings of the National Academy of Sciences of the United States of America 2007 Jun 26;104(26):10835-40
Proceedings of the National Academy of Sciences of the United States of America 2007 Jun 26;104(26):10835-40
The APC tumor suppressor counteracts beta-catenin activation and H3K4 methylation at Wnt target genes.
Sierra J, Yoshida T, Joazeiro CA, Jones KA
Genes & development 2006 Mar 1;20(5):586-600
Genes & development 2006 Mar 1;20(5):586-600
Myc-binding-site recognition in the human genome is determined by chromatin context.
Guccione E, Martinato F, Finocchiaro G, Luzi L, Tizzoni L, Dall' Olio V, Zardo G, Nervi C, Bernard L, Amati B
Nature cell biology 2006 Jul;8(7):764-70
Nature cell biology 2006 Jul;8(7):764-70
Genome-wide analysis of menin binding provides insights into MEN1 tumorigenesis.
Scacheri PC, Davis S, Odom DT, Crawford GE, Perkins S, Halawi MJ, Agarwal SK, Marx SJ, Spiegel AM, Meltzer PS, Collins FS
PLoS genetics 2006 Apr;2(4):e51
PLoS genetics 2006 Apr;2(4):e51
Histone and DNA methylation defects at Hox genes in mice expressing a SET domain-truncated form of Mll.
Terranova R, Agherbi H, Boned A, Meresse S, Djabali M
Proceedings of the National Academy of Sciences of the United States of America 2006 Apr 25;103(17):6629-34
Proceedings of the National Academy of Sciences of the United States of America 2006 Apr 25;103(17):6629-34
Physical association and coordinate function of the H3 K4 methyltransferase MLL1 and the H4 K16 acetyltransferase MOF.
Dou Y, Milne TA, Tackett AJ, Smith ER, Fukuda A, Wysocka J, Allis CD, Chait BT, Hess JL, Roeder RG
Cell 2005 Jun 17;121(6):873-85
Cell 2005 Jun 17;121(6):873-85
Menin and MLL cooperatively regulate expression of cyclin-dependent kinase inhibitors.
Milne TA, Hughes CM, Lloyd R, Yang Z, Rozenblatt-Rosen O, Dou Y, Schnepp RW, Krankel C, Livolsi VA, Gibbs D, Hua X, Roeder RG, Meyerson M, Hess JL
Proceedings of the National Academy of Sciences of the United States of America 2005 Jan 18;102(3):749-54
Proceedings of the National Academy of Sciences of the United States of America 2005 Jan 18;102(3):749-54
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: KMT2A/MLL Antibody [NB600-248] - Samples: Nuclear extract (50 and 15 ug) from HeLa, 293T, and Jurkat cells. Antibody: Affinity purified rabbit anti-MLL1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: KMT2A/MLL Antibody [NB600-248] - Samples: Nuclear Extract (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MLL1 antibody NB600-248 used for IP at 6 ug per reaction. MLL1 was also immunoprecipitated by rabbit anti-MLL1 antibody NB600-249. For blotting immunoprecipitated MLL1, NB600-248 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
