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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- F49231 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- XRCC1 Antibody
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 407-435 from the human protein was used as the immunogen for this XRCC1 antibody.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the XRCC1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- XRCC1 antibody western blot analysis in A375 lysate. Predicted molecular weight ~69 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of A549 cells using XRCC1 antibody at 1:25. Alexa Fluor 488-conjugated secondary was used (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 conjugated Phalloidin (red).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- XRCC1 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- XRCC1 antibody flow cytometric analysis of A375 cells (green) compared to a negative control (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
