MA5-13412

antibody from Invitrogen Antibodies

Targeting: XRCC1 RCC

Provider product page for MA5-13412

Western blot

Immunoprecipitation

Immunohistochemistry

Other assay

Antibody data

Antibody Data
Antigen structure
References [26]
Comments [0]
Validations
Western blot [1]
Immunohistochemistry [2]
Other assay [5]

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Product number: MA5-13412 - Provider product page
Provider: Invitrogen Antibodies
Product name: XRCC1 Monoclonal Antibody (33-2-5)
Antibody type: Monoclonal
Antigen: Recombinant full-length protein
Description: MA5-13412 targets XRCC1 in IHC (P), IP, and WB applications and shows reactivity with Human and Rat samples. The MA5-13412 immunogen is recombinant human XRCC1 protein.
Reactivity: Human, Rat
Host: Mouse
Isotype: IgG
Antibody clone number: 33-2-5
Vial size: 500 µL
Concentration: 0.2 mg/mL
Storage: 4° C

XRCC1 protein structure - MA5-13412 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunoprecipitation of XRCC1 using XRCC1 Monoclonal Antibody (Product # MA5-13412) on Native Human LS174T Cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig 5 FHA domain of PNKP is involved in distribution to subnuclear compartments. (A) Confirmation of protein expression of GFP-PNKP constructs in U2OS cells by western blot analysis. (B) Interaction between FL PNKP and PNKP mutants (R35A, K138A and R35A/K138A) examined by immunoprecipitation. GFP-Trap magnetic agarose beads were used for immunoprecipitation from GFP-tagged PNKP expressing HEK293 cells. XRCC1 and XRCC4 were detected by western blotting and GFP-tagged PNKP was detected by Ponceau staining. (C) Representative live-cell images of GFP-tagged PNKP mutants, expressing in U2OS cells, with DIC images. Scale bar indicates as 10mum. (D, E, F) Dot plot of the N/C ratio of GFI. At least 150 cells were analyzed and plotted. (G) GFP-tagged FL PNKP and R35A PNKP localization in nucleus. Subnuclear regions with increased or decreased GFI are surrounded by white or black dashed circles, respectively. (H) Representative live-cell images of GFP-tagged FL PNKP, R35A PNKP and mCherry-tagged Fibrillarin localization in nucleolus. (I) Dot plot of the nucleous/nucleus ratio of GFI. At least 150 cells were analyzed and plotted. ns: not significant (p > 0.05); **: 0.005 < p

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Involvement of hnRNP UL1 in the DDR in human nucleoli. (A) The DNA damage sensitivity of HEK UL1 KO cells compared to HEK WT cells was tested after 2.5 h of treatment with the genotoxic reagents ETO and CPT. After this time, the cells were harvested at the following time points: 18 h, 24 h, 30 h, 48 h, 72 h and 80 h. Cell viability was assessed by Trypan blue staining, and the percentage of survival was calculated. The results were normalized to those for the control cells treated with DMSO. (B) DNA damage was assessed by Comet Assay in HEK UL1 KO cells compared to HEK WT cells after DNA damage induced with ETO and CPT. Cells treated with DMSO were used as negative controls. For the assay, 50 cells per sample were analyzed. The error bars represent the SDs of three biological replicates. The p values were calculated using Student's t test, and the statistical significance is represented as follows: *P

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Interaction between Chk2 and XRCC1. ( A ) In vivo association of Chk2 with XRCC1. 293T cells were co-transfected with Myc-tagged Chk2 and/or His-tagged XRCC1 , then whole-cell extracts were subjected to immunoprecipitation (IP) with anti-His antibody and the immunoprecipitated proteins analysed by immunoblotting (IB) with anti-Myc or anti-His antibodies (upper panel). The lower panel shows IB of the whole-cell extract. ( B ) Association of Chk2 and XRCC1 is damage-inducible. 293T cells were either untreated or treated for 1 h with increasing concentrations of methyl methanesulphonate (MMS), then whole-cell lysates were subjected to IP with anti-Chk2 antibody and the immunoprecipitated proteins were analysed on IB using anti-XRCC1 or anti-Chk2 antibodies (upper panel). The lower panel shows IB of the whole-cell extract for Chk2, XRCC1, and phosphorylated p53 (p53-pS15), an indicator of DNA damage. ( C ) In vivo association of endogenous Chk2 with endogenous XRCC1 is induced by alkylation (MMS), oxidative damage (H 2 O 2 ), or ionizing radiation (IR) (upper panel). Whole-cell lysates were analysed in the lower panel as in (B). ( D ) Constructs of GST-fused XRCC1 segments used in the in vitro binding assay. ( E ) Upper panel, purified Flag-tagged Chk2 produced in bacteria was incubated with purified GST-XRCC1 fragments, and the proteins pulled down by glutathione beads were analysed by IB with anti-Flag antibody. Lower panel, pull down of GST-XRCC1 fusion proteins was confirmed

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig 6 XRCC1 recruitment following 355 nm laser microirradiation in TNBC cell lines. Microirradiation with a 355 nm laser occurred and cells were fixed at the indicated time points and processed for immunofluorescence of XRCC1. Quantification over time is shown on the left with a representative image from 0.5 min shown on the right for A) MDA-157, B) MDA-231, C) HCC1806, and D) MDA-468. A minimum of 18 cells were irradiated over three separate experiments and quantified as described in the materials and methods section and reported as XRCC1 Foci Intensity in arbitrary units (a.u.). Scale bar = 20 mum.