NB100-541

antibody from Novus Biologicals

Targeting: XRCC1 RCC

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB100-541

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-541, RRID:AB_10002908

Product name

Rabbit Polyclonal XRCC1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. NB100-541 is specific for human phospho-XRCC1 protein.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Concentration

1.0 mg/ml

Storage

Store at 4C. Do not freeze.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: XRCC1 [p Ser485, p Thr488] Antibody [NB100-541] - Whole cell lysate (50 ug) from asynchronously growing HeLa (H), 293T (T) and mouse NIH-3T3 (M) cells. Antibody used at 0.1 ug/ml. The binding of the antibody was blocked by pre-incubation with the phospho-peptide but was not blocked by pre-incubation with

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: XRCC1 [p Ser485, p Thr488] Antibody [NB100-541] - See Internal Notes B

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: XRCC1 [p Ser485, p Thr488] Antibody [NB100-541] - See Internal Notes

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: XRCC1 [p Ser485, p Thr488] Antibody [NB100-541] - Sample: FFPE sections of human stomach carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti- Phospho-XRCC1-S485/T488 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB