15817-1-AP

antibody from Invitrogen Antibodies

Targeting: XRCC4

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

15817-1-AP

Provider

Invitrogen Antibodies

Product name

XRCC4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: SRIHLVSEP SITHFLQVSW EKTLESGFVI TLTDGHSAWT GTVSESEISQ EADDMAMEKG KYVGELRKAL LSGAGPADVY TFNFSKESCY FFFEKNLKDV SFRLGSFNLE KVENPAEVIR ELICYCLDTI AENQAKNEHL QKENERLLRD WNDVQGRFEK CVSAKEALET DLYKRFILVL NEKKTKIRSL HNKLLNAAQE REKDIKQEGE TAICSEMTAD RDPVYDESTD EESENQTDLS GLASAAVSKD DSIISSLDVT DIAPSRKRRQ RMQRNLGTEP KMAPQENQLQ EKEKGRKKET SEKEAV (6-310 aa encoded by B C005259)

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.28 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

COLO 320 cells were subjected to SDS PAGE followed by western blot with 15817-1-AP (XRCC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 15817-1-AP (XRCC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 15817-1-AP ( XRCC4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded rat small intestine using 15817-1-AP (XRCC4 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded rat small intestine using 15817-1-AP (XRCC4 antibody) at dilution of 1:100 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded rat testis using 15817-1-AP (XRCC4 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded rat testis using 15817-1-AP (XRCC4 antibody) at dilution of 1:100 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 15817-1-AP (XRCC4 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 15817-1-AP (XRCC4 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-XRCC4 (IP:15817-1-AP, 4ug; Detection:15817-1-AP 1:800) with HepG2 cells lysate 3200ug.