Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-22044 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- XRCC4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: HeLa nuclear extract. Predicted reactivity: Chimpanzee (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of XRCC4 using 30 µg of A) Jurkat (B) K562 and C) HL-60 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a XRCC4 polyclonal antibody (Product # PA5-22044) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of XRCC4 was performed by separating 30 µg of HeLa whole cell and nuclear extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a XRCC4 Polyclonal Antibody (Product # PA5-22044) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using XRCC4 Polyclonal Antibody (Product # PA5-22044). HeLa whole cell and nuclear extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with XRCC4 Polyclonal Antibody (Product # PA5-22044) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of XRCC4 in paraformaldehyde-fixed HeLa cells using a XRCC4 polyclonal antibody (Product # PA5-22044) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using XRCC4 (Product # PA5-22044) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- XRCC4 Polyclonal Antibody detects XRCC4 protein at nucleus on HBL435 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HBL435 xenograft. XRCC4 Polyclonal Antibody (Product # PA5-22044) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
