- HPA006801 - Atlas Antibodies XRCC4 antibody

Submitted references Organization and dynamics of the nonhomologous end-joining machinery during DNA double-strand break repair.

Reid DA, Keegan S, Leo-Macias A, Watanabe G, Strande NT, Chang HH, Oksuz BA, Fenyo D, Lieber MR, Ramsden DA, Rothenberg E
Proceedings of the National Academy of Sciences of the United States of America 2015 May 19;112(20):E2575-84

Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells

Stadler C, Rexhepaj E, Singan V, Murphy R, Pepperkok R, Uhlén M, Simpson J, Lundberg E
Nature Methods 2013 February;10(4):315-323

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Enhanced validation

Submitted by: klas2
Enhanced method: Genetic validation
Main image
Experimental details: Western blot of cell lysate from U-2 OS cells transfected with either siRNA targeting XRCC4 or control siRNA. Lane 1: Marker (250, 130, 95, 72, 55, 36, 28, 17, 10) Lane 2: Cell lysate from U-2OS cells transfected with siRNA targeting XRCC4 Lane 3: N/A Lane 4: Cell lysate from U-2OS cells transfected with control siRNA Right image, lane 1-4: loading control
Sample type: U-2 OS
Primary Ab dilution: 1:142
Conjugate: Horseradish Peroxidase
Secondary Ab: Secondary Ab
Secondary Ab dilution: 1:3000
Knockdown/Genetic Approaches Application: Western blot
KD Reagent Type: siRNA
Downregulation: >50%
KD Reagent Prod page: KD Reagent Prod page
KD Reagent Prod no: s14949
KD Reagent Provider: Thermo Fisher Scientific
KD Reagent Prod no 2: s14950
KD Reagent Page 2: KD Reagent Page 2
KD Reagent Prod no 3: s14951
KD Reagent Page 3: KD Reagent Page 3
Antibody Lot Number: R07495
Appendix: 56b1cf89ae7f3.pdf

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Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Western blot analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-XRCC4 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Western blot analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-XRCC4 antibody. Remaining relative intensity is presented

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Recombinant expression validation
Main image
Experimental details: Western blot analysis in control (vector only transfected HEK293T lysate) and XRCC4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402930).

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus.
Sample type: HUMAN

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Orthogonal validation
Main image
Experimental details: Immunohistochemistry analysis in human testis and liver tissues using Anti-XRCC4 antibody. Corresponding XRCC4 RNA-seq data are presented for the same tissues.
Sample type: HUMAN
Orthogonal method: Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human lymph node shows strong nuclear positivity in germinal center cells,.

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human testis shows high expression.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human liver shows low expression as expected.
Sample type: HUMAN

HPA006801

Antibody data