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LearnPA1-970
antibody from Invitrogen Antibodies
Targeting: PSMC5
p45, p45/SUG, S8, SUG-1, SUG1, TBP10, TRIP1
Western blot
Immunoprecipitation
Other assayAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Other assay [1]
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- Product number
- PA1-970 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-PSMC5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- PA1-971 detects proteasome 19S subunit S10B from human cells. PA1-971 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects a 42 kDa protein representing proteasome 19S subunit S10B from HeLa cell lysate. This antibody detects, to a lesser extent, an ~30 kDa protein which could correspond to subunit degradation product. PA1-971 antigen is recombinant human proteasome 19S subunit S10B.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- Lot Dependent
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Degradation of promoter-bound p65/RelA is essential for the prompt termination of the nuclear factor kappaB response.
Saccani S, Marazzi I, Beg AA, Natoli G
The Journal of experimental medicine 2004 Jul 5;200(1):107-13
The Journal of experimental medicine 2004 Jul 5;200(1):107-13
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- IP-MS enrichment of PSMC5 (LFQ intensity): PSMC5 was enriched 297-fold from MCF7 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Product # 90409) and PSMC5 antibody (Product # PA1-970). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity.
