Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX101777 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX101777, RRID:AB_1950269
- Product name
- FEN1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Inhibition of hydrogen sulfide biosynthesis sensitizes lung adenocarcinoma to chemotherapeutic drugs by inhibiting mitochondrial DNA repair and suppressing cellular bioenergetics.
Szczesny B, Marcatti M, Zatarain JR, Druzhyna N, Wiktorowicz JE, Nagy P, Hellmich MR, Szabo C
Scientific reports 2016 Nov 3;6:36125
Scientific reports 2016 Nov 3;6:36125
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded A549 xenograft, using FEN1(GTX101777) antibody at 1:100 dilution.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: HepG2 (GTX27900) 10% SDS PAGE GTX101777 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- FEN1 antibody detects FEN1 protein by western blot analysis. A. 30 ?g NIH-3T3 whole cell lysate/extract B. 30 ?g JC whole cell lysate/extract C. 30 ?g BCL-1 whole cell lysate/extract D. 30 ?g Raw264.7 whole cell lysate/extract 10% SDS-PAGE FEN1 antibody (GTX101777) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- FEN1 antibody detects FEN1 protein by western blot analysis. A. 30 ?g PC-12 whole cell lysate/extract 10% SDS-PAGE FEN1 antibody (GTX101777) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed A549, using FEN1(GTX101777) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- FEN1 antibody immunoprecipitates FEN1 protein in IP experiments.IP samples: Jurkat whole cell extractA. 30 £gg Jurkat whole cell extractB. Control with 4 £gg of preimmune Rabbit IgGC. Immunoprecipitation of FEN1 protein by 4 £gg FEN1 antibody (GTX101777)10 % SDS-PAGEThe immunoprecipitated FEN1 protein was detected by FEN1 antibody (GTX101777) diluted at 1:500.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- FEN1 antibody detects FEN1 protein at nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. FEN1 antibody (GTX101777) diluted at 1:500.