Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
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Validation data
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- Product number
- LS-B12760 - Provider product page
- Provider
- LSBio
- Product name
- IHC-plus™ FEN1 Antibody (aa250-300) LS-B12760
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store at 2-8°C for up to one year.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Detection of Human Fen1 by Western Blot. Samples: Whole cell lysate (20 ug) from HeLa cells separated on a 4-12% PAGE gel. Antibodies: Affinity purified rabbit anti-Fen1 used at the indicated concentrations. Secondary antibody was goat anti-Rabbit HRP conjugate used at 1:40000 (for 0.015 ug/ml anti-Fen1) or 1:60000 (for 1 and 0.1 ug/ml anti-Fen1). Detection: ECL with the indicated exposure.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Detection of Human Fen1 by Western Blot. Samples: Whole cell lysate (20 ug) from HeLa cells separated on a 4-12% PAGE gel. Antibodies: Affinity purified rabbit anti-Fen1 used at the indicated concentrations. Secondary antibody was goat anti-Rabbit HRP conjugate used at 1:40000 (for 0.015 ug/ml anti-Fen1) or 1:60000 (for 1 and 0.1 ug/ml anti-Fen1). Detection: ECL with the indicated exposure.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Detection of human Fen1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Fen1 antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.