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Western blot
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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [4]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
- Other assay [2]
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- Product number
- PA5-88244 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FEN1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: GDVLQNEEGE TTSHLMGMFY RTIRMMENGI KPVYVFDGKP PQLKSGELAK RSERRAEAEK QLQQAQAAGA EQEVEKFTKR LVKVTKQHND ECKHLLSLMG IPYLDAPSEA EASCAALVKA GKVYAAATED MDCLTFGSPV LMRHLTASEA KKLPIQEFHL SRILQELGLN QEQFVDLCIL LGSDYCESIR GIGPKRAVDL IQKHKSIEEI VRRLDPNKYP VPENWLHKEA HQLFLEPEVL DPESVELKWS EPNEEELIKF MCGEKQFSEE RIRSGVKRLS KSRQGSTQGR LDDFFKVTGS LSSAKRKEPE PKGSTKKKAK TGAAGKFKRG K
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.96 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of FEN1 was performed in A549 cells using FEN1 Polyclonal Antibody (Product # PA5-88244).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of FEN1 was performed in HeLa cells using FEN1 Polyclonal Antibody (Product # PA5-88244).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of FEN1 in A549 cells. Samples were incubated with FEN1 Polyclonal antibody (Product # PA5-88244).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of FEN1 in HeLa cells. Samples were incubated with FEN1 Polyclonal antibody (Product # PA5-88244).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of FEN1 in 200 μg extracts of HeLa cells. Samples were precipitated with 1 μg FEN1 Polyclonal antibody (Product # PA5-88244). Western blot was performed from the immunoprecipitate using FEN1 Polyclonal antibody (Product # PA5-88244) at a dilution of 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of FEN1 in paraffin-embedded human lung cancer. Samples were incubated with FEN1 Polyclonal antibody (Product # PA5-88244) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of FEN1 in paraffin-embedded mouse liver. Samples were incubated with FEN1 Polyclonal antibody (Product # PA5-88244) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of FEN1 was performed in 200 µg extracts of HeLa cells using FEN1 Polyclonal Antibody (Product # PA5-88244). Western blot was performed from the immunoprecipitate using FEN1 Polyclonal Antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of FEN1 was performed in 200 µg extracts of HeLa cells using FEN1 Polyclonal Antibody (Product # PA5-88244). Western blot was performed from the immunoprecipitate using FEN1 Polyclonal Antibody.
