A301-231A-T

antibody from Invitrogen Antibodies

Targeting: POLH RAD30A, XP-V

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A301-231A-T

Provider

Invitrogen Antibodies

Product name

PolH Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

10 µL

Concentration

200 µg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human PolH by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-PolH antibody (Product # A301-231A; lot A301-231A-3) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human PolH by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PolH antibody A301-231A (lot A301-231A-2) used for IP at 3 µg per reaction. PolH was also immunoprecipitated by rabbit anti-PolH antibody A301-230A. For blotting immunoprecipitated PolH, A301-231A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse PolH by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PolH antibody A301-231A (lot A301-231A-2) used for WB at 0.2 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human PolH by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Hela cells prepared using NETN lysis buffer. Antibodies: Affinity purified Rabbit anti-PolH antibody (Product # A301-231A; lot A301-231A-3) used for IP at 6 µg per reaction. PolH was also immunoprecipitated by a previous lot of this antibody (lot A301-231A-2) and rabbit anti-PolH antibody (Product # A301-230A). For blotting immunoprecipitated PolH, Product # A301-231A was used at 0.1 µg/mL. Chemiluminescence with an exposure time of 3 minutes.