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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- LS-C775073 - Provider product page
- Provider
- LSBio
- Product name
- GRK5 Antibody (clone S145-20, Atto 700) LS-C775073
- Antibody type
- Monoclonal
- Description
- Protein G purified
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Near infrared dye
- Isotype
- IgG
- Antibody clone number
- S145-20
- Storage
- Store at -20°C.
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Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Western Blot analysis of Monkey COS cells transfected with untagged GRK6 showing detection of ~65 kDa GRK (pan) protein using Mouse Anti-GRK (pan) Monoclonal Antibody, Clone S145-20. Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with untagged GRK6. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-GRK (pan) Monoclonal Antibody at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~65 kDa.
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GRK (pan) Monoclonal Antibody, Clone S145-20. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GRK (pan) Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GRK (pan) Antibody. (D) Composite.
