MA5-27695

antibody from Invitrogen Antibodies

Targeting: GRK5 GPRK5

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-27695

Provider

Invitrogen Antibodies

Product name

Anti-GRK5 Monoclonal Antibody (S145-20)

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

S145-20

Vial size

100 µg

Concentration

1 mg/ml

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of GRK5 in monkey COS cells transfected with untagged GRK6 with 15 µg of sample. The sample was blocked with 2% BSA and 2% skim milk in TBST, incubated with GRK5 monoclonal antibody (Product # MA5-27695) using a dilution of 1:200 (16 hours at RT), followed by Goat Anti-Mouse HRP (1 hour at RT) at a dilution of 1:1000 and ECL development (6 min at RT). Samples were arranged as follows: Lane 1: Molecular Weight Ladder, Lane 2: Monkey COS cells transfected with untagged GRK6.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of GRK5 in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with GRK5 monoclonal antibody (Product # MA5-27695) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:100, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) GRK (pan) Antibody, D) Merged image. Magnification: 60x.