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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- TA322773 - Provider product page
- Provider
- OriGene
- Product name
- Anti-BAG3 rabbit polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Anti-BAG3 rabbit polyclonal antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- BAG3
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µl
- Concentration
- 0.9mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Predicted band size: 62kDa. Positive control: Mouse muscle tissue, K562 cell and mouse heart tissue lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lane1: Mouse muscle tissue lysate Lane2: K562 cell lysate Lane3: Mouse heart tissue lysate Lysates: 40 ug per lane Primary antibody: 1/450 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 10 seconds)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Predicted cell location: Cytoplasm. Positive control: Human lung cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human lung cancer tissue using BAG3 antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification:x200)
- Validation comment
- IHC
