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ELISA
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunoprecipitation [1]
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Validation data
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- Product number
- LS-C372042 - Provider product page
- Provider
- LSBio
- Product name
- BAG3 / BAG-3 Antibody (aa2-577) LS-C372042
- Antibody type
- Polyclonal
- Description
- Caprylic acid and ammonium sulfate precipitation
- Reactivity
- Mouse
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.
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Supportive validation
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- LSBio (provider)
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- Experimental details
- Immunoprecipitating Bag3 in K562 whole cell lysate Lane 1: Rabbit control IgG (1µg) instead of Bag3 Antibody in K562 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: Bag3 Antibody (6µg) + K562 whole cell lysate (500µg) Lane 3: K562 whole cell lysate (10µg)
